Evaluation of methicillin resistance in Staphylococcus intermedius isolated from dogs

V. Meucci; M. Vanni; Luca Guardabassi; Arshnee Moodley; G. Soldani; L. Intorre

doi:10.1007/s11259-010-9395-0

Evaluation of methicillin resistance in Staphylococcus intermedius isolated from dogs

V. Meucci, M. Vanni, Luca Guardabassi, Arshnee Moodley, G. Soldani, L. Intorre

8 Citations (Scopus)

Abstract

Methicillin and multi-drug resistance were investigated in 136 Staphylococcus intermedius strains of canine origin. The large majority of isolates were found to be mecA-negative by polymerase chain reaction, whereas only four strains were positive for the mecA gene. All mecA-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. A significantly higher resistance to fusidic acid, lincosamides, and cotrimoxazole was observed in methicillin-resistant S. intermedius (MRSI) compared with methicillinsusceptible strains. Although the prevalence of MRSI in dogs appeared to be low, methicillin resistance was confirmed to be associated with multi-drug resistance, suggesting the importance of antimicrobial susceptibility testing of canine S. intermedius strains.

Original language	English
Journal	Veterinary Research Communications
Volume	34
Issue number	Suppl 1
Pages (from-to)	S79-S82
Number of pages	4
ISSN	0165-7380
DOIs	https://doi.org/10.1007/s11259-010-9395-0
Publication status	Published - Jun 2010

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Former LIFE faculty

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10.1007/s11259-010-9395-0

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title = "Evaluation of methicillin resistance in Staphylococcus intermedius isolated from dogs",

abstract = "Methicillin and multi-drug resistance were investigated in 136 Staphylococcus intermedius strains of canine origin. The large majority of isolates were found to be mecA-negative by polymerase chain reaction, whereas only four strains were positive for the mecA gene. All mecA-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. A significantly higher resistance to fusidic acid, lincosamides, and cotrimoxazole was observed in methicillin-resistant S. intermedius (MRSI) compared with methicillinsusceptible strains. Although the prevalence of MRSI in dogs appeared to be low, methicillin resistance was confirmed to be associated with multi-drug resistance, suggesting the importance of antimicrobial susceptibility testing of canine S. intermedius strains.",

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author = "V. Meucci and M. Vanni and Luca Guardabassi and Arshnee Moodley and G. Soldani and L. Intorre",

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TY - JOUR

T1 - Evaluation of methicillin resistance in Staphylococcus intermedius isolated from dogs

AU - Meucci, V.

AU - Vanni, M.

AU - Guardabassi, Luca

AU - Moodley, Arshnee

AU - Soldani, G.

AU - Intorre, L.

PY - 2010/6

Y1 - 2010/6

N2 - Methicillin and multi-drug resistance were investigated in 136 Staphylococcus intermedius strains of canine origin. The large majority of isolates were found to be mecA-negative by polymerase chain reaction, whereas only four strains were positive for the mecA gene. All mecA-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. A significantly higher resistance to fusidic acid, lincosamides, and cotrimoxazole was observed in methicillin-resistant S. intermedius (MRSI) compared with methicillinsusceptible strains. Although the prevalence of MRSI in dogs appeared to be low, methicillin resistance was confirmed to be associated with multi-drug resistance, suggesting the importance of antimicrobial susceptibility testing of canine S. intermedius strains.

AB - Methicillin and multi-drug resistance were investigated in 136 Staphylococcus intermedius strains of canine origin. The large majority of isolates were found to be mecA-negative by polymerase chain reaction, whereas only four strains were positive for the mecA gene. All mecA-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. A significantly higher resistance to fusidic acid, lincosamides, and cotrimoxazole was observed in methicillin-resistant S. intermedius (MRSI) compared with methicillinsusceptible strains. Although the prevalence of MRSI in dogs appeared to be low, methicillin resistance was confirmed to be associated with multi-drug resistance, suggesting the importance of antimicrobial susceptibility testing of canine S. intermedius strains.

KW - Former LIFE faculty

KW - Antimicrobials

KW - Dog

KW - Methicillin resistance

KW - Staphylococci

U2 - 10.1007/s11259-010-9395-0

DO - 10.1007/s11259-010-9395-0

M3 - Journal article

C2 - 20437274

SN - 0165-7380

VL - 34

SP - S79-S82

JO - Veterinary Research Communications

JF - Veterinary Research Communications

IS - Suppl 1

ER -

Evaluation of methicillin resistance in Staphylococcus intermedius isolated from dogs

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