Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry

Riccardo Zenezini Chiozzi; Anna Laura Capriotti; Chiara Cavaliere; Francesca Ferraris; Giorgia La Barbera; Susy Piovesana; Aldo Laganà

doi:10.1002/jssc.201700135

Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry

Riccardo Zenezini Chiozzi, Anna Laura Capriotti, Chiara Cavaliere, Francesca Ferraris, Giorgia La Barbera^*, Susy Piovesana, Aldo Laganà

^*Corresponding author for this work

Nutrition and Health

15 Citations (Scopus)

Abstract

Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 μm sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions. In this work, an evaluation of four chromatographic systems, with either a single or two in-series Kinetex™ C₁₈ columns, either packed with 2.6 or 1.7 μm particles, is presented for the targeted analysis of a standard mixture and the untargeted analysis of a strawberry extract. An ultra-high performance chromatographic system coupled via an electrospray source to a hybrid quadrupole-Orbitrap mass spectrometer was used. From the extensive comparison, a surprising result was obtained, namely, that the system identifying the largest number of features was the one with two in-series connected columns with the larger particle size. The inconsistency among the theoretical assumptions and the applicative findings points out the importance of an extensive chromatographic evaluation for the comprehensive untargeted profiling of complex real samples.

Original language	English
Journal	Journal of Separation Science
Volume	40
Issue number	12
Pages (from-to)	2541-2557
Number of pages	17
ISSN	1615-9306
DOIs	https://doi.org/10.1002/jssc.201700135
Publication status	Published - Jun 2017

Keywords

core-shell particles
high-resolution mass spectrometry
strawberry
untargeted profiling

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10.1002/jssc.201700135

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Zenezini Chiozzi, R., Capriotti, A. L., Cavaliere, C., Ferraris, F., La Barbera, G., Piovesana, S., & Laganà, A. (2017). Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry. Journal of Separation Science, 40(12), 2541-2557. https://doi.org/10.1002/jssc.201700135

Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry. / Zenezini Chiozzi, Riccardo; Capriotti, Anna Laura; Cavaliere, Chiara et al.
In: Journal of Separation Science, Vol. 40, No. 12, 06.2017, p. 2541-2557.

Research output: Contribution to journal › Journal article › Research › peer-review

Zenezini Chiozzi, R, Capriotti, AL, Cavaliere, C, Ferraris, F, La Barbera, G, Piovesana, S & Laganà, A 2017, 'Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry', Journal of Separation Science, vol. 40, no. 12, pp. 2541-2557. https://doi.org/10.1002/jssc.201700135

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AU - La Barbera, Giorgia

AU - Piovesana, Susy

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AB - Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 μm sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions. In this work, an evaluation of four chromatographic systems, with either a single or two in-series Kinetex™ C18 columns, either packed with 2.6 or 1.7 μm particles, is presented for the targeted analysis of a standard mixture and the untargeted analysis of a strawberry extract. An ultra-high performance chromatographic system coupled via an electrospray source to a hybrid quadrupole-Orbitrap mass spectrometer was used. From the extensive comparison, a surprising result was obtained, namely, that the system identifying the largest number of features was the one with two in-series connected columns with the larger particle size. The inconsistency among the theoretical assumptions and the applicative findings points out the importance of an extensive chromatographic evaluation for the comprehensive untargeted profiling of complex real samples.

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Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry

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