TY - JOUR
T1 - Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry
AU - Zenezini Chiozzi, Riccardo
AU - Capriotti, Anna Laura
AU - Cavaliere, Chiara
AU - Ferraris, Francesca
AU - La Barbera, Giorgia
AU - Piovesana, Susy
AU - Laganà, Aldo
PY - 2017/6
Y1 - 2017/6
N2 - Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 μm sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions. In this work, an evaluation of four chromatographic systems, with either a single or two in-series Kinetex™ C18 columns, either packed with 2.6 or 1.7 μm particles, is presented for the targeted analysis of a standard mixture and the untargeted analysis of a strawberry extract. An ultra-high performance chromatographic system coupled via an electrospray source to a hybrid quadrupole-Orbitrap mass spectrometer was used. From the extensive comparison, a surprising result was obtained, namely, that the system identifying the largest number of features was the one with two in-series connected columns with the larger particle size. The inconsistency among the theoretical assumptions and the applicative findings points out the importance of an extensive chromatographic evaluation for the comprehensive untargeted profiling of complex real samples.
AB - Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 μm sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions. In this work, an evaluation of four chromatographic systems, with either a single or two in-series Kinetex™ C18 columns, either packed with 2.6 or 1.7 μm particles, is presented for the targeted analysis of a standard mixture and the untargeted analysis of a strawberry extract. An ultra-high performance chromatographic system coupled via an electrospray source to a hybrid quadrupole-Orbitrap mass spectrometer was used. From the extensive comparison, a surprising result was obtained, namely, that the system identifying the largest number of features was the one with two in-series connected columns with the larger particle size. The inconsistency among the theoretical assumptions and the applicative findings points out the importance of an extensive chromatographic evaluation for the comprehensive untargeted profiling of complex real samples.
KW - core-shell particles
KW - high-resolution mass spectrometry
KW - strawberry
KW - untargeted profiling
UR - http://www.scopus.com/inward/record.url?scp=85019896731&partnerID=8YFLogxK
U2 - 10.1002/jssc.201700135
DO - 10.1002/jssc.201700135
M3 - Journal article
C2 - 28448690
AN - SCOPUS:85019896731
SN - 1615-9306
VL - 40
SP - 2541
EP - 2557
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 12
ER -