EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

Yixin Dong; Kyo Ichi Isono; Kazuyuki Ohbo; Takaho A. Endo; Osamu Ohara; Mamiko Maekawa; Yoshiro Toyama; Chizuru Ito; Kiyotaka Toshimori; Kristian Helin; Narumi Ogonuki; Kimiko Inoue; Atsuo Ogura; Kazutsune Yamagata; Issay Kitabayashi; Haruhiko Koseki

doi:10.1128/mcb.00082-17

EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

Yixin Dong^*, Kyo Ichi Isono, Kazuyuki Ohbo, Takaho A. Endo, Osamu Ohara, Mamiko Maekawa, Yoshiro Toyama, Chizuru Ito, Kiyotaka Toshimori, Kristian Helin, Narumi Ogonuki, Kimiko Inoue, Atsuo Ogura, Kazutsune Yamagata, Issay Kitabayashi, Haruhiko Koseki

^*Corresponding author for this work

The Danish Stem Cell Center

12 Citations (Scopus)

Abstract

Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation- mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

Original language	English
Article number	e00082-17
Journal	Molecular and Cellular Biology
Volume	37
Issue number	19
Number of pages	16
ISSN	0270-7306
DOIs	https://doi.org/10.1128/mcb.00082-17
Publication status	Published - 1 Oct 2017

Keywords

EPC1
Histone acetylation
Mouse
Spermatids
Spermiogenesis
TIP60

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Dong, Y., Isono, K. I., Ohbo, K., Endo, T. A., Ohara, O., Maekawa, M., Toyama, Y., Ito, C., Toshimori, K., Helin, K., Ogonuki, N., Inoue, K., Ogura, A., Yamagata, K., Kitabayashi, I., & Koseki, H. (2017). EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice. Molecular and Cellular Biology, 37(19), [e00082-17]. https://doi.org/10.1128/mcb.00082-17

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abstract = "Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation- mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.",

keywords = "EPC1, Histone acetylation, Mouse, Spermatids, Spermiogenesis, TIP60",

author = "Yixin Dong and Isono, {Kyo Ichi} and Kazuyuki Ohbo and Endo, {Takaho A.} and Osamu Ohara and Mamiko Maekawa and Yoshiro Toyama and Chizuru Ito and Kiyotaka Toshimori and Kristian Helin and Narumi Ogonuki and Kimiko Inoue and Atsuo Ogura and Kazutsune Yamagata and Issay Kitabayashi and Haruhiko Koseki",

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T1 - EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

AU - Dong, Yixin

AU - Isono, Kyo Ichi

AU - Ohbo, Kazuyuki

AU - Endo, Takaho A.

AU - Ohara, Osamu

AU - Maekawa, Mamiko

AU - Toyama, Yoshiro

AU - Ito, Chizuru

AU - Toshimori, Kiyotaka

AU - Helin, Kristian

AU - Ogonuki, Narumi

AU - Inoue, Kimiko

AU - Ogura, Atsuo

AU - Yamagata, Kazutsune

AU - Kitabayashi, Issay

AU - Koseki, Haruhiko

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation- mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

AB - Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation- mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

KW - EPC1

KW - Histone acetylation

KW - Mouse

KW - Spermatids

KW - Spermiogenesis

KW - TIP60

U2 - 10.1128/mcb.00082-17

DO - 10.1128/mcb.00082-17

M3 - Journal article

C2 - 28694333

AN - SCOPUS:85029209048

SN - 0270-7306

VL - 37

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 19

M1 - e00082-17

ER -

EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

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Keywords

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