EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

Yixin Dong; Kyo Ichi Isono; Kazuyuki Ohbo; Takaho A. Endo; Osamu Ohara; Mamiko Maekawa; Yoshiro Toyama; Chizuru Ito; Kiyotaka Toshimori; Kristian Helin; Narumi Ogonuki; Kimiko Inoue; Atsuo Ogura; Kazutsune Yamagata; Issay Kitabayashi; Haruhiko Koseki

doi:10.1128/mcb.00082-17

EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

Yixin Dong^*, Kyo Ichi Isono, Kazuyuki Ohbo, Takaho A. Endo, Osamu Ohara, Mamiko Maekawa, Yoshiro Toyama, Chizuru Ito, Kiyotaka Toshimori, Kristian Helin, Narumi Ogonuki, Kimiko Inoue, Atsuo Ogura, Kazutsune Yamagata, Issay Kitabayashi, Haruhiko Koseki

^*Corresponding author af dette arbejde

DanStem

12 Citationer (Scopus)

Abstract

Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation-mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

Originalsprog	Engelsk
Artikelnummer	e00082-17
Tidsskrift	Molecular and Cellular Biology
Vol/bind	37
Udgave nummer	19
Antal sider	16
ISSN	0270-7306
DOI	https://doi.org/10.1128/mcb.00082-17
Status	Udgivet - 1 okt. 2017

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10.1128/mcb.00082-17

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Dong, Y., Isono, K. I., Ohbo, K., Endo, T. A., Ohara, O., Maekawa, M., Toyama, Y., Ito, C., Toshimori, K., Helin, K., Ogonuki, N., Inoue, K., Ogura, A., Yamagata, K., Kitabayashi, I., & Koseki, H. (2017). EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice. Molecular and Cellular Biology, 37(19), [e00082-17]. https://doi.org/10.1128/mcb.00082-17

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title = "EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice",

abstract = "Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation- mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.",

keywords = "EPC1, Histone acetylation, Mouse, Spermatids, Spermiogenesis, TIP60",

author = "Yixin Dong and Isono, {Kyo Ichi} and Kazuyuki Ohbo and Endo, {Takaho A.} and Osamu Ohara and Mamiko Maekawa and Yoshiro Toyama and Chizuru Ito and Kiyotaka Toshimori and Kristian Helin and Narumi Ogonuki and Kimiko Inoue and Atsuo Ogura and Kazutsune Yamagata and Issay Kitabayashi and Haruhiko Koseki",

year = "2017",

month = oct,

day = "1",

doi = "10.1128/mcb.00082-17",

language = "English",

volume = "37",

journal = "Molecular and Cellular Biology",

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TY - JOUR

T1 - EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

AU - Dong, Yixin

AU - Isono, Kyo Ichi

AU - Ohbo, Kazuyuki

AU - Endo, Takaho A.

AU - Ohara, Osamu

AU - Maekawa, Mamiko

AU - Toyama, Yoshiro

AU - Ito, Chizuru

AU - Toshimori, Kiyotaka

AU - Helin, Kristian

AU - Ogonuki, Narumi

AU - Inoue, Kimiko

AU - Ogura, Atsuo

AU - Yamagata, Kazutsune

AU - Kitabayashi, Issay

AU - Koseki, Haruhiko

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation- mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

AB - Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation- mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

KW - EPC1

KW - Histone acetylation

KW - Mouse

KW - Spermatids

KW - Spermiogenesis

KW - TIP60

U2 - 10.1128/mcb.00082-17

DO - 10.1128/mcb.00082-17

M3 - Journal article

C2 - 28694333

AN - SCOPUS:85029209048

SN - 0270-7306

VL - 37

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 19

M1 - e00082-17

ER -

EPC1/TIP60-mediated histone acetylation facilitates spermiogenesis in mice

Abstract

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