Enhanced sensitization and elicitation responses caused by mixtures of common fragrance allergens

TY - JOUR

T1 - Enhanced sensitization and elicitation responses caused by mixtures of common fragrance allergens

AU - Bonefeld, Charlotte Menné

AU - Nielsen, Morten Milek

AU - Rubin, Ingrid Maria Cecilia

AU - Vennegaard, Marie Torp

AU - Dabelsteen, Sally

AU - Gimenéz-Arnau, Elena

AU - Lepoittevin, Jean-Pierre

AU - Geisler, Carsten

AU - Johansen, Jeanne Duus

N1 - © 2011 John Wiley & Sons A/S.

PY - 2011/12

Y1 - 2011/12

N2 - Background. Perfumes are complex mixtures composed of many fragrance ingredients, many of which are known to be only weak allergens when tested individually. It is therefore surprising that fragrance contact allergy is one of the most common forms of contact allergy. Objectives. To investigate whether mixing different fragrance allergens leads to increased sensitization potency, and to examine the difference in the challenge response to one chemical in mice sensitized either with the mixture of allergens or with only the relevant allergen. Methods. CBA mice were sensitized with three different concentrations of three fragrance allergens alone or as a mixture. The sensitization and elicitation responses were measured by ear thickness plus infiltration of B and T cells and T cell proliferation in the draining lymph nodes. Results. We found a dose-dependent sensitization response for each of the allergens. An increased response was seen when the allergens were mixed. A stronger challenge response to cinnamal was seen in mice sensitized with the allergen mixture than in mice sensitized with cinnamal alone. Conclusions. Our findings suggest that mixtures of allergens increase the primary response that potentiates the generation of memory T cells in response to the specific allergen. Thus, allergen mixtures enhance both induction and elicitation of contact allergy.

AB - Background. Perfumes are complex mixtures composed of many fragrance ingredients, many of which are known to be only weak allergens when tested individually. It is therefore surprising that fragrance contact allergy is one of the most common forms of contact allergy. Objectives. To investigate whether mixing different fragrance allergens leads to increased sensitization potency, and to examine the difference in the challenge response to one chemical in mice sensitized either with the mixture of allergens or with only the relevant allergen. Methods. CBA mice were sensitized with three different concentrations of three fragrance allergens alone or as a mixture. The sensitization and elicitation responses were measured by ear thickness plus infiltration of B and T cells and T cell proliferation in the draining lymph nodes. Results. We found a dose-dependent sensitization response for each of the allergens. An increased response was seen when the allergens were mixed. A stronger challenge response to cinnamal was seen in mice sensitized with the allergen mixture than in mice sensitized with cinnamal alone. Conclusions. Our findings suggest that mixtures of allergens increase the primary response that potentiates the generation of memory T cells in response to the specific allergen. Thus, allergen mixtures enhance both induction and elicitation of contact allergy.

KW - Acrolein

KW - Aldehydes

KW - Allergens

KW - Animals

KW - CD4 Lymphocyte Count

KW - CD8-Positive T-Lymphocytes

KW - Cell Proliferation

KW - Cells, Cultured

KW - Cyclohexenes

KW - Dermatitis, Allergic Contact

KW - Dose-Response Relationship, Immunologic

KW - Eugenol

KW - Female

KW - Flow Cytometry

KW - Mice

KW - Mice, Inbred CBA

KW - Perfume

U2 - 10.1111/j.1600-0536.2011.01945.x

DO - 10.1111/j.1600-0536.2011.01945.x

M3 - Journal article

C2 - 21767274

SN - 0105-1873

VL - 65

SP - 336

EP - 342

JO - Contact Dermatitis

JF - Contact Dermatitis

IS - 6

ER -