Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release.

TY - JOUR

T1 - Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release.

AU - Verstreken, Patrik

AU - Kjaerulff, Ole

AU - Lloyd, Thomas E

AU - Atkinson, Richard

AU - Zhou, Yi

AU - Meinertzhagen, Ian A

AU - Bellen, Hugo J

N1 - Keywords: Adaptor Proteins, Signal Transducing; Animals; Carrier Proteins; Cell Size; Clathrin; Drosophila melanogaster; Endocytosis; Female; Gene Expression Regulation, Developmental; Male; Microscopy, Electron; Motor Neurons; Mutation; Nervous System; Neuromuscular Junction; Neurotransmitter Agents; Presynaptic Terminals; Protein Transport; Synaptic Membranes; Synaptic Transmission; Synaptic Vesicles

PY - 2002

Y1 - 2002

N2 - We have identified mutations in Drosophila endophilin to study its function in vivo. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo. Mutant larvae that lack Endophilin fail to take up FM1-43 dye in synaptic boutons, indicating an inability to retrieve synaptic membrane. This defect is accompanied by an expansion of the presynaptic membrane, and a depletion of vesicles from the bouton lumen. Interestingly, mutant larvae are still able to sustain release at 15%-20% of the normal rate during high-frequency stimulation. We propose that kiss-and-run maintains neurotransmission at active zones of the larval NMJ in endophilin animals.

AB - We have identified mutations in Drosophila endophilin to study its function in vivo. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo. Mutant larvae that lack Endophilin fail to take up FM1-43 dye in synaptic boutons, indicating an inability to retrieve synaptic membrane. This defect is accompanied by an expansion of the presynaptic membrane, and a depletion of vesicles from the bouton lumen. Interestingly, mutant larvae are still able to sustain release at 15%-20% of the normal rate during high-frequency stimulation. We propose that kiss-and-run maintains neurotransmission at active zones of the larval NMJ in endophilin animals.

M3 - Journal article

C2 - 11955450

SN - 0092-8674

VL - 109

SP - 101

EP - 112

JO - Cell

JF - Cell

IS - 1

ER -