@article{4c246600752011dd8d9f000ea68e967b,
title = "Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release.",
abstract = "We have identified mutations in Drosophila endophilin to study its function in vivo. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo. Mutant larvae that lack Endophilin fail to take up FM1-43 dye in synaptic boutons, indicating an inability to retrieve synaptic membrane. This defect is accompanied by an expansion of the presynaptic membrane, and a depletion of vesicles from the bouton lumen. Interestingly, mutant larvae are still able to sustain release at 15%-20% of the normal rate during high-frequency stimulation. We propose that kiss-and-run maintains neurotransmission at active zones of the larval NMJ in endophilin animals.",
author = "Patrik Verstreken and Ole Kjaerulff and Lloyd, {Thomas E} and Richard Atkinson and Yi Zhou and Meinertzhagen, {Ian A} and Bellen, {Hugo J}",
note = "Keywords: Adaptor Proteins, Signal Transducing; Animals; Carrier Proteins; Cell Size; Clathrin; Drosophila melanogaster; Endocytosis; Female; Gene Expression Regulation, Developmental; Male; Microscopy, Electron; Motor Neurons; Mutation; Nervous System; Neuromuscular Junction; Neurotransmitter Agents; Presynaptic Terminals; Protein Transport; Synaptic Membranes; Synaptic Transmission; Synaptic Vesicles",
year = "2002",
language = "English",
volume = "109",
pages = "101--12",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",
}