Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing

TY - JOUR

T1 - Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing

AU - Ibrahim, M E

AU - Hag-Ali, M

AU - el-Hassan, A M

AU - Theander, T G

AU - Kharazmi, A

N1 - Keywords: Animals; Antimony Sodium Gluconate; Cell Line; Drug Resistance; Humans; Leishmania donovani; Leishmania major; Leishmaniasis; Leishmaniasis, Cutaneous; Leishmaniasis, Visceral; Macrophages; Mice; Sudan

PY - 1994

Y1 - 1994

N2 - A total of 17 Leishmania isolates, 6 of them isolated from antimony-resistant patients, were collected in the Sudan and tested for their sensitivity to sodium stibogluconate (Pentostam) as promastigotes. Six of those isolates were tested as amastigotes infecting a murine macrophage cell line. The results indicated that the conventional promastigote screening assay did not correlate with the clinical picture, whereas the amastigote/macrophage system produced results that pertained to the in vivo responses to the drug. A laboratory-generated resistant strain of L. major was adapted to grow at a high concentration of Pentostam (1000 micrograms/ml) as promastigotes but was quite sensitive to the drug at much lower concentrations in the amastigote/(macrophage system (20 micrograms/ml), thus suggesting that Pentostam's inhibitory action is mediated through the macrophage rather than through a direct toxic effect exerted on the parasite.

AB - A total of 17 Leishmania isolates, 6 of them isolated from antimony-resistant patients, were collected in the Sudan and tested for their sensitivity to sodium stibogluconate (Pentostam) as promastigotes. Six of those isolates were tested as amastigotes infecting a murine macrophage cell line. The results indicated that the conventional promastigote screening assay did not correlate with the clinical picture, whereas the amastigote/macrophage system produced results that pertained to the in vivo responses to the drug. A laboratory-generated resistant strain of L. major was adapted to grow at a high concentration of Pentostam (1000 micrograms/ml) as promastigotes but was quite sensitive to the drug at much lower concentrations in the amastigote/(macrophage system (20 micrograms/ml), thus suggesting that Pentostam's inhibitory action is mediated through the macrophage rather than through a direct toxic effect exerted on the parasite.

M3 - Journal article

C2 - 7855121

SN - 0932-0113

VL - 80

SP - 569

EP - 574

JO - Parasitology Reseach

JF - Parasitology Reseach

IS - 7

ER -