Coxiella burnetti associated placental lesions and infection level in parturient cows

Mette Sif Hansen; Annie Rodolakis; Denis Cochonneau; Jens Frederik Gramstrup Agger; Anna-Bodil Christoffersen; Tim Kåre Jensen; Jørgen Steen Agerholm

doi:10.1016/j.tvjl.2010.12.021

Coxiella burnetti associated placental lesions and infection level in parturient cows

Mette Sif Hansen, Annie Rodolakis, Denis Cochonneau, Jens Frederik Gramstrup Agger, Anna-Bodil Christoffersen, Tim Kåre Jensen, Jørgen Steen Agerholm

43 Citations (Scopus)

Abstract

Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.

Original language	English
Journal	The Veterinary Journal
Volume	190
Issue number	2
Pages (from-to)	e135-e139
Number of pages	5
ISSN	1090-0233
DOIs	https://doi.org/10.1016/j.tvjl.2010.12.021
Publication status	Published - Nov 2011

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title = "Coxiella burnetti associated placental lesions and infection level in parturient cows",

abstract = "Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.",

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language = "English",

volume = "190",

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T1 - Coxiella burnetti associated placental lesions and infection level in parturient cows

AU - Hansen, Mette Sif

AU - Rodolakis, Annie

AU - Cochonneau, Denis

AU - Agger, Jens Frederik Gramstrup

AU - Christoffersen, Anna-Bodil

AU - Jensen, Tim Kåre

AU - Agerholm, Jørgen Steen

PY - 2011/11

Y1 - 2011/11

N2 - Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.

AB - Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.

U2 - 10.1016/j.tvjl.2010.12.021

DO - 10.1016/j.tvjl.2010.12.021

M3 - Journal article

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VL - 190

SP - e135-e139

JO - The Veterinary Journal

JF - The Veterinary Journal

IS - 2

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Coxiella burnetti associated placental lesions and infection level in parturient cows

Abstract

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