Coxiella burnetti associated placental lesions and infection level in parturient cows

Mette Sif Hansen; Annie Rodolakis; Denis Cochonneau; Jens Frederik Gramstrup Agger; Anna-Bodil Christoffersen; Tim Kåre Jensen; Jørgen Steen Agerholm

doi:10.1016/j.tvjl.2010.12.021

Coxiella burnetti associated placental lesions and infection level in parturient cows

Mette Sif Hansen, Annie Rodolakis, Denis Cochonneau, Jens Frederik Gramstrup Agger, Anna-Bodil Christoffersen, Tim Kåre Jensen, Jørgen Steen Agerholm

43 Citationer (Scopus)

Abstract

Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.

Originalsprog	Engelsk
Tidsskrift	The Veterinary Journal
Vol/bind	190
Udgave nummer	2
Sider (fra-til)	e135-e139
Antal sider	5
ISSN	1090-0233
DOI	https://doi.org/10.1016/j.tvjl.2010.12.021
Status	Udgivet - nov. 2011

Adgang til dokumentet

10.1016/j.tvjl.2010.12.021

Citationsformater

@article{2187ce8334574c909b472a5ccd053246,

title = "Coxiella burnetti associated placental lesions and infection level in parturient cows",

abstract = "Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.",

author = "Hansen, {Mette Sif} and Annie Rodolakis and Denis Cochonneau and Agger, {Jens Frederik Gramstrup} and Anna-Bodil Christoffersen and Jensen, {Tim K{\aa}re} and Agerholm, {J{\o}rgen Steen}",

year = "2011",

month = nov,

doi = "10.1016/j.tvjl.2010.12.021",

language = "English",

volume = "190",

pages = "e135--e139",

journal = "The Veterinary Journal",

issn = "1090-0233",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Coxiella burnetti associated placental lesions and infection level in parturient cows

AU - Hansen, Mette Sif

AU - Rodolakis, Annie

AU - Cochonneau, Denis

AU - Agger, Jens Frederik Gramstrup

AU - Christoffersen, Anna-Bodil

AU - Jensen, Tim Kåre

AU - Agerholm, Jørgen Steen

PY - 2011/11

Y1 - 2011/11

N2 - Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.

AB - Cotyledons (n= 170) from dairy cattle were analysed for Coxiella burnetii by real-time (rt) PCR targeting the IS1111a and icd genes. Positive cases (n= 90) and a random selection of negative cases (n= 20) were examined by histology, immunohistochemistry and, if infection level was high, by fluorescence in situ hybridisation. PCR results were compared to bulk tank milk (BTM) antibody levels. Placental infection was detected in cows from herds at all BTM antibody levels. However the likelihood of placental infection was generally higher in herds with intermediate or high BMT antibody levels than in herds with low antibody levels. Histological examination revealed a range of mostly mild cotyledonary changes; C. burnetii infection was only rarely associated with inflammation. This may explain why bovine Q fever is usually not clinically apparent. Nevertheless, infected cattle will shed C. burnetii at calving and this can occur even in herds without BTM antibodies.

U2 - 10.1016/j.tvjl.2010.12.021

DO - 10.1016/j.tvjl.2010.12.021

M3 - Journal article

SN - 1090-0233

VL - 190

SP - e135-e139

JO - The Veterinary Journal

JF - The Veterinary Journal

IS - 2

ER -

Coxiella burnetti associated placental lesions and infection level in parturient cows

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater