Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes

TY - JOUR

T1 - Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes

AU - Barfod, Lea

AU - Nielsen, Morten A

AU - Turner, Louise

AU - Dahlbäck, Madeleine

AU - Jensen, Anja T R

AU - Hviid, Lars

AU - Theander, Thor G

AU - Salanti, Ali

N1 - Keywords: Animals; Antigens, Protozoan; Baculoviridae; Erythrocytes; Female; Genetic Vectors; Humans; Immune Sera; Immunoglobulin G; Mice; Plasmodium falciparum; Rabbits; Recombinant Proteins

PY - 2006

Y1 - 2006

N2 - We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

AB - We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

U2 - 10.1128/IAI.01617-05

DO - 10.1128/IAI.01617-05

M3 - Journal article

C2 - 16790811

SN - 0019-9567

VL - 74

SP - 4357

EP - 4360

JO - Infection and Immunity

JF - Infection and Immunity

IS - 7

ER -