Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans: A Randomized Double-Blind Placebo-Controlled Trial

Dorien Reijnders; Gijs H Goossens; Gerben D A Hermes; Evelien P J G Neis; Christina M van der Beek; Jasper Most; Jens J Holst; Kaatje Lenaerts; Ruud S Kootte; Max Nieuwdorp; Albert K Groen; Steven W M Olde Damink; Mark V Boekschoten; Hauke Smidt; Erwin G Zoetendal; Cornelis H C Dejong; Ellen E Blaak

doi:10.1016/j.cmet.2016.06.016

Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans: A Randomized Double-Blind Placebo-Controlled Trial

Dorien Reijnders, Gijs H Goossens, Gerben D A Hermes, Evelien P J G Neis, Christina M van der Beek, Jasper Most, Jens J Holst, Kaatje Lenaerts, Ruud S Kootte, Max Nieuwdorp, Albert K Groen, Steven W M Olde Damink, Mark V Boekschoten, Hauke Smidt, Erwin G Zoetendal, Cornelis H C Dejong, Ellen E Blaak

152 Citations (Scopus)

Abstract

The gut microbiota has been implicated in obesity and cardiometabolic diseases, although evidence in humans is scarce. We investigated how gut microbiota manipulation by antibiotics (7-day administration of amoxicillin, vancomycin, or placebo) affects host metabolism in 57 obese, prediabetic men. Vancomycin, but not amoxicillin, decreased bacterial diversity and reduced Firmicutes involved in short-chain fatty acid and bile acid metabolism, concomitant with altered plasma and/or fecal metabolite concentrations. Adipose tissue gene expression of oxidative pathways was upregulated by antibiotics, whereas immune-related pathways were downregulated by vancomycin. Antibiotics did not affect tissue-specific insulin sensitivity, energy/substrate metabolism, postprandial hormones and metabolites, systemic inflammation, gut permeability, and adipocyte size. Importantly, energy harvest, adipocyte size, and whole-body insulin sensitivity were not altered at 8-week follow-up, despite a still considerably altered microbial composition, indicating that interference with adult microbiota by 7-day antibiotic treatment has no clinically relevant impact on metabolic health in obese humans.

Original language	English
Journal	Cell Metabolism
Volume	24
Issue number	1
Pages (from-to)	63-74
Number of pages	12
ISSN	1550-4131
DOIs	https://doi.org/10.1016/j.cmet.2016.06.016
Publication status	Published - 12 Jul 2016

Keywords

Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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http://www.cell.com/article/S1550413116303023/pdf

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Reijnders, D., Goossens, G. H., Hermes, G. D. A., Neis, E. P. J. G., van der Beek, C. M., Most, J., Holst, J. J., Lenaerts, K., Kootte, R. S., Nieuwdorp, M., Groen, A. K., Olde Damink, S. W. M., Boekschoten, M. V., Smidt, H., Zoetendal, E. G., Dejong, C. H. C., & Blaak, E. E. (2016). Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans: A Randomized Double-Blind Placebo-Controlled Trial. Cell Metabolism, 24(1), 63-74. https://doi.org/10.1016/j.cmet.2016.06.016

Reijnders, D, Goossens, GH, Hermes, GDA, Neis, EPJG, van der Beek, CM, Most, J, Holst, JJ, Lenaerts, K, Kootte, RS, Nieuwdorp, M, Groen, AK, Olde Damink, SWM, Boekschoten, MV, Smidt, H, Zoetendal, EG, Dejong, CHC & Blaak, EE 2016, 'Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans: A Randomized Double-Blind Placebo-Controlled Trial', Cell Metabolism, vol. 24, no. 1, pp. 63-74. https://doi.org/10.1016/j.cmet.2016.06.016

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Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans: A Randomized Double-Blind Placebo-Controlled Trial

Abstract

Keywords

UN SDGs

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