Effector and regulatory T cells in patients with acute optic neuritis

Anna Tsakiri; Erik Kjærsgaard; Nikolaos Grigoriadis; Inge Marie Svane; Jette L Frederiksen

doi:10.1159/000330242

Effector and regulatory T cells in patients with acute optic neuritis

Anna Tsakiri, Erik Kjærsgaard, Nikolaos Grigoriadis, Inge Marie Svane, Jette L Frederiksen

3 Citations (Scopus)

Abstract

Objective: Optic neuritis (ON) is an autoimmune acute demyelinating disease of the optic nerve and may occur in patients with confirmed multiple sclerosis (MS) or as a clinically isolated syndrome. T lymphocytes play a central role in the pathogenesis of MS. The phenotype of different T cell subsets is usually characterized by the expression of distinct cell surface receptors such as CD45RA, CD45RO, CCR7, CD27 and CD28. The aim of this study was to characterize the phenotype of distinct subsets of CD4 and CD8 T cells in patients with isolated ON. Methods: CD4 and CD8 T cell subsets were characterized by flow cytometry in fresh peripheral blood and cerebrospinal fluid (CSF) samples using the surface markers CD27, CD25, CD45RA, CD45RO and intracellular FOXP3. The T cell subsets expressed in patients with acute ON (n = 64; symptom onset of ON within the preceding 28 days) were compared to those of a gender-and age-matched healthy control (HC) group (n = 32). Results: Both CD4 ⁺ and CD8 ⁺ naïve T cells in the ON group were significantly reduced in the CSF. In contrast, most of the intermediate-stage and late effector CD4 ⁺ and CD8 ⁺ T cell subsets as well as the CD4 ⁺ T regulatory cells were expressed in ON patients, though not at all in the CSF of the HC group. Conclusion: These results add important evidence for inflammatory and regulatory activity in ON and early MS.

Original language	English
Journal	Neuroimmunomodulation
Volume	19
Issue number	2
Pages (from-to)	111-20
Number of pages	10
ISSN	1021-7401
DOIs	https://doi.org/10.1159/000330242
Publication status	Published - Jan 2012

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@article{fb4335efb3de4a9fb446fea28780f2b6,

title = "Effector and regulatory T cells in patients with acute optic neuritis",

abstract = "Objective: Optic neuritis (ON) is an autoimmune acute demyelinating disease of the optic nerve and may occur in patients with confirmed multiple sclerosis (MS) or as a clinically isolated syndrome. T lymphocytes play a central role in the pathogenesis of MS. The phenotype of different T cell subsets is usually characterized by the expression of distinct cell surface receptors such as CD45RA, CD45RO, CCR7, CD27 and CD28. The aim of this study was to characterize the phenotype of distinct subsets of CD4 and CD8 T cells in patients with isolated ON. Methods: CD4 and CD8 T cell subsets were characterized by flow cytometry in fresh peripheral blood and cerebrospinal fluid (CSF) samples using the surface markers CD27, CD25, CD45RA, CD45RO and intracellular FOXP3. The T cell subsets expressed in patients with acute ON (n = 64; symptom onset of ON within the preceding 28 days) were compared to those of a gender-and age-matched healthy control (HC) group (n = 32). Results: Both CD4 + and CD8 + na{\"i}ve T cells in the ON group were significantly reduced in the CSF. In contrast, most of the intermediate-stage and late effector CD4 + and CD8 + T cell subsets as well as the CD4 + T regulatory cells were expressed in ON patients, though not at all in the CSF of the HC group. Conclusion: These results add important evidence for inflammatory and regulatory activity in ON and early MS.",

author = "Anna Tsakiri and Erik Kj{\ae}rsgaard and Nikolaos Grigoriadis and Svane, {Inge Marie} and Frederiksen, {Jette L}",

note = "Copyright {\textcopyright} 2012 S. Karger AG, Basel.",

year = "2012",

month = jan,

doi = "10.1159/000330242",

language = "English",

volume = "19",

pages = "111--20",

journal = "NeuroImmunoModulation",

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T1 - Effector and regulatory T cells in patients with acute optic neuritis

AU - Tsakiri, Anna

AU - Kjærsgaard, Erik

AU - Grigoriadis, Nikolaos

AU - Svane, Inge Marie

AU - Frederiksen, Jette L

PY - 2012/1

Y1 - 2012/1

N2 - Objective: Optic neuritis (ON) is an autoimmune acute demyelinating disease of the optic nerve and may occur in patients with confirmed multiple sclerosis (MS) or as a clinically isolated syndrome. T lymphocytes play a central role in the pathogenesis of MS. The phenotype of different T cell subsets is usually characterized by the expression of distinct cell surface receptors such as CD45RA, CD45RO, CCR7, CD27 and CD28. The aim of this study was to characterize the phenotype of distinct subsets of CD4 and CD8 T cells in patients with isolated ON. Methods: CD4 and CD8 T cell subsets were characterized by flow cytometry in fresh peripheral blood and cerebrospinal fluid (CSF) samples using the surface markers CD27, CD25, CD45RA, CD45RO and intracellular FOXP3. The T cell subsets expressed in patients with acute ON (n = 64; symptom onset of ON within the preceding 28 days) were compared to those of a gender-and age-matched healthy control (HC) group (n = 32). Results: Both CD4 + and CD8 + naïve T cells in the ON group were significantly reduced in the CSF. In contrast, most of the intermediate-stage and late effector CD4 + and CD8 + T cell subsets as well as the CD4 + T regulatory cells were expressed in ON patients, though not at all in the CSF of the HC group. Conclusion: These results add important evidence for inflammatory and regulatory activity in ON and early MS.

AB - Objective: Optic neuritis (ON) is an autoimmune acute demyelinating disease of the optic nerve and may occur in patients with confirmed multiple sclerosis (MS) or as a clinically isolated syndrome. T lymphocytes play a central role in the pathogenesis of MS. The phenotype of different T cell subsets is usually characterized by the expression of distinct cell surface receptors such as CD45RA, CD45RO, CCR7, CD27 and CD28. The aim of this study was to characterize the phenotype of distinct subsets of CD4 and CD8 T cells in patients with isolated ON. Methods: CD4 and CD8 T cell subsets were characterized by flow cytometry in fresh peripheral blood and cerebrospinal fluid (CSF) samples using the surface markers CD27, CD25, CD45RA, CD45RO and intracellular FOXP3. The T cell subsets expressed in patients with acute ON (n = 64; symptom onset of ON within the preceding 28 days) were compared to those of a gender-and age-matched healthy control (HC) group (n = 32). Results: Both CD4 + and CD8 + naïve T cells in the ON group were significantly reduced in the CSF. In contrast, most of the intermediate-stage and late effector CD4 + and CD8 + T cell subsets as well as the CD4 + T regulatory cells were expressed in ON patients, though not at all in the CSF of the HC group. Conclusion: These results add important evidence for inflammatory and regulatory activity in ON and early MS.

U2 - 10.1159/000330242

DO - 10.1159/000330242

M3 - Journal article

C2 - 22248727

SN - 1021-7401

VL - 19

SP - 111

EP - 120

JO - NeuroImmunoModulation

JF - NeuroImmunoModulation

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Effector and regulatory T cells in patients with acute optic neuritis

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