Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae

Thomas Ask Aabo; Ivan R. Perch-Nielsen; Jeppe Seidelin Dam; Darwin Palima; Henrik Siegumfeldt; Jesper Glückstad; Nils Arneborg

doi:10.1117/1.3430731

Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae

Thomas Ask Aabo, Ivan R. Perch-Nielsen, Jeppe Seidelin Dam, Darwin Palima, Henrik Siegumfeldt, Jesper Glückstad, Nils Arneborg

19 Citations (Scopus)

Abstract

The effect of a 1070-nm continuous and pulsed wave ytterbium
fiber laser on the growth of Saccharomyces cerevisiae single
cells is investigated over a time span of 4 to 5 h. The cells are subjected
to optical traps consisting of two counterpropagating plane
wave beams with a uniform flux along the x, y axis. Even at the lowest
continuous power investigated—i.e., 0.7 mW—the growth of S. cerevisiae
cell clusters is markedly inhibited. The minimum power required
to successfully trap single S. cerevisiae cells in three dimensions
is estimated to be 3.5 mW. No threshold power for the
photodamage, but instead a continuous response to the increased accumulated
dose is found in the regime investigated from
0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant
and varying the exposure time and power—i.e. pulsing—we find that
the growth of S. cerevisiae cells is increasingly inhibited with increasing
power. These results indicate that growth of S. cerevisiae is dependent
on both the power as well as the accumulated dose at
1070 nm.

Original language	English
Journal	Journal of Biomedical Optics
Volume	15
Issue number	4
Number of pages	7
ISSN	1083-3668
DOIs	https://doi.org/10.1117/1.3430731
Publication status	Published - Jul 2010

Keywords

Former LIFE faculty

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10.1117/1.3430731

http://dx.doi.org/10.1117/1.3430731

Cite this

@article{a22a14f6d5b04bd192afb99ee4406690,

title = "Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae",

abstract = "The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated—i.e., 0.7 mW—the growth of S. cerevisiae cell clusters is markedly inhibited. The minimum power required to successfully trap single S. cerevisiae cells in three dimensions is estimated to be 3.5 mW. No threshold power for the photodamage, but instead a continuous response to the increased accumulated dose is found in the regime investigated from 0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant and varying the exposure time and power—i.e. pulsing—we find that the growth of S. cerevisiae cells is increasingly inhibited with increasing power. These results indicate that growth of S. cerevisiae is dependent on both the power as well as the accumulated dose at 1070 nm.",

keywords = "Former LIFE faculty",

author = "Aabo, {Thomas Ask} and Perch-Nielsen, {Ivan R.} and Dam, {Jeppe Seidelin} and Darwin Palima and Henrik Siegumfeldt and Jesper Gl{\"u}ckstad and Nils Arneborg",

note = "Special Section on Selected Topics in Biophotonics: Photodynamic Therapy and Optical Micromanipulation for Biophotonics",

year = "2010",

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doi = "10.1117/1.3430731",

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TY - JOUR

T1 - Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae

AU - Aabo, Thomas Ask

AU - Perch-Nielsen, Ivan R.

AU - Dam, Jeppe Seidelin

AU - Palima, Darwin

AU - Siegumfeldt, Henrik

AU - Glückstad, Jesper

AU - Arneborg, Nils

N1 - Special Section on Selected Topics in Biophotonics: Photodynamic Therapy and Optical Micromanipulation for Biophotonics

PY - 2010/7

Y1 - 2010/7

N2 - The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated—i.e., 0.7 mW—the growth of S. cerevisiae cell clusters is markedly inhibited. The minimum power required to successfully trap single S. cerevisiae cells in three dimensions is estimated to be 3.5 mW. No threshold power for the photodamage, but instead a continuous response to the increased accumulated dose is found in the regime investigated from 0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant and varying the exposure time and power—i.e. pulsing—we find that the growth of S. cerevisiae cells is increasingly inhibited with increasing power. These results indicate that growth of S. cerevisiae is dependent on both the power as well as the accumulated dose at 1070 nm.

AB - The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated—i.e., 0.7 mW—the growth of S. cerevisiae cell clusters is markedly inhibited. The minimum power required to successfully trap single S. cerevisiae cells in three dimensions is estimated to be 3.5 mW. No threshold power for the photodamage, but instead a continuous response to the increased accumulated dose is found in the regime investigated from 0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant and varying the exposure time and power—i.e. pulsing—we find that the growth of S. cerevisiae cells is increasingly inhibited with increasing power. These results indicate that growth of S. cerevisiae is dependent on both the power as well as the accumulated dose at 1070 nm.

KW - Former LIFE faculty

U2 - 10.1117/1.3430731

DO - 10.1117/1.3430731

M3 - Journal article

C2 - 20799783

SN - 1083-3668

VL - 15

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 4

ER -