EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

Sebastian Virreira Winter; Florian Meier; Christoph Wichmann; Juergen Cox; Matthias Mann; Felix Meissner

doi:10.1038/s41592-018-0037-8

EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

Sebastian Virreira Winter, Florian Meier, Christoph Wichmann, Juergen Cox, Matthias Mann, Felix Meissner

32 Citations (Scopus)

Abstract

We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.

Original language	English
Journal	Nature Methods
Volume	15
Issue number	7
Pages (from-to)	527-530
Number of pages	4
ISSN	1548-7091
DOIs	https://doi.org/10.1038/s41592-018-0037-8
Publication status	Published - 2018

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abstract = "We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.",

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AU - Virreira Winter, Sebastian

AU - Meier, Florian

AU - Wichmann, Christoph

AU - Cox, Juergen

AU - Mann, Matthias

AU - Meissner, Felix

PY - 2018

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AB - We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.

U2 - 10.1038/s41592-018-0037-8

DO - 10.1038/s41592-018-0037-8

M3 - Journal article

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SN - 1548-7091

VL - 15

SP - 527

EP - 530

JO - Nature Methods

JF - Nature Methods

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EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

Abstract

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