EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

TY - JOUR

T1 - EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

AU - Virreira Winter, Sebastian

AU - Meier, Florian

AU - Wichmann, Christoph

AU - Cox, Juergen

AU - Mann, Matthias

AU - Meissner, Felix

PY - 2018

Y1 - 2018

N2 - We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.

AB - We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.

U2 - 10.1038/s41592-018-0037-8

DO - 10.1038/s41592-018-0037-8

M3 - Journal article

C2 - 29915187

SN - 1548-7091

VL - 15

SP - 527

EP - 530

JO - Nature Methods

JF - Nature Methods

IS - 7

ER -