Does Pseudomonas produce terpenes? Characterization of terpene synthases from Pseudomonas

TY - CONF

T1 - Does Pseudomonas produce terpenes? Characterization of terpene synthases from Pseudomonas

AU - Podduturi, Raju

AU - Hennessy, Rosanna Catherine

AU - Petersen, Mikael Agerlin

AU - Stougaard, Peter

AU - Jørgensen, Niels O. G.

PY - 2018

Y1 - 2018

N2 - Terpenes account for one of major class of natural products with a great potential in pharma, flavor and fragrance industrial applications. It has been well known about terpene compounds originate from plant and fungal sources. In recent times the terpenes of bacterial origin has been explored; bacterial groups belongs to actinomycetes, cyanobacteria and myxobacteria are known to produce structurally diverse terpenes. Pseudomonas spp are known to bio transform terpenes but so far there are no reports that shows biosynthesis of terpenes from this genus except for Chou et al., 2011. In which authors have demonstrated recombinant expression of terpene synthase from Pseudomonas fluorescens PfO-1. Here we would like to explore potentially new group of terpene producing gram negative bacteria. Protein cluster exploration form NCBI data base has revealed presence of four different terpene synthase clusters in Pseudomonas. Among these protein clusters, the cluster PCLA_903025 is homologous to a well characterized bifunctional enzyme geosmin synthase, which involves in biosynthesis of geosmin. Geosmin is a sesquiterpene alcohol with an earthy flavor note and it is one of major off-flavor compound in drinking water, cultured fish and wine. The objective of current study is to demonstrate native and recombinant expression of terpene synthases from Pseudomonas agarici (LMG 02116) and Pseudomonas gingeri (LMG 05327).

AB - Terpenes account for one of major class of natural products with a great potential in pharma, flavor and fragrance industrial applications. It has been well known about terpene compounds originate from plant and fungal sources. In recent times the terpenes of bacterial origin has been explored; bacterial groups belongs to actinomycetes, cyanobacteria and myxobacteria are known to produce structurally diverse terpenes. Pseudomonas spp are known to bio transform terpenes but so far there are no reports that shows biosynthesis of terpenes from this genus except for Chou et al., 2011. In which authors have demonstrated recombinant expression of terpene synthase from Pseudomonas fluorescens PfO-1. Here we would like to explore potentially new group of terpene producing gram negative bacteria. Protein cluster exploration form NCBI data base has revealed presence of four different terpene synthase clusters in Pseudomonas. Among these protein clusters, the cluster PCLA_903025 is homologous to a well characterized bifunctional enzyme geosmin synthase, which involves in biosynthesis of geosmin. Geosmin is a sesquiterpene alcohol with an earthy flavor note and it is one of major off-flavor compound in drinking water, cultured fish and wine. The objective of current study is to demonstrate native and recombinant expression of terpene synthases from Pseudomonas agarici (LMG 02116) and Pseudomonas gingeri (LMG 05327).

UR - https://dechema.converia.de/frontend/index.php?folder_id=1585

M3 - Poster

T2 - Bioflavour 2018 - Biotechnology of Flavours, Fragrances and Functional Inngredients

Y2 - 18 September 2018

ER -