DNA repair phenotype and dietary antioxidant supplementation

Serena Guarnieri; Steffen Loft; Patrizia Riso; Marisa Porrini; Lotte Risom; Henrik Enghusen Poulsen; Lars Ove Dragsted; Peter Møller

doi:10.1017/S0007114507842796

DNA repair phenotype and dietary antioxidant supplementation

Serena Guarnieri, Steffen Loft, Patrizia Riso, Marisa Porrini, Lotte Risom, Henrik Enghusen Poulsen, Lars Ove Dragsted, Peter Møller

42 Citations (Scopus)

Abstract

Phytochemicals may protect cellular DNA by direct antioxidant effect or modulation of the DNA repair activity. We investigated the repair activity towards oxidised DNA in human mononuclear blood cells (MNBC) in two placebo-controlled antioxidant intervention studies as follows: (1) well-nourished subjects who ingested 600 g fruits and vegetables, or tablets containing the equivalent amount of vitamins and minerals, for 24 d; (2) poorly nourished male smokers who ingested 500 mg vitamin C/d as slow- or plain-release formulations together with 182 mg vitamin E/d for 4 weeks. The mean baseline levels of DNA repair incisions were 65.2 (95 % CI 60.4, 70.0) and 86.1 (95 % CI 76.2, 99.9) among the male smokers and well-nourished subjects, respectively. The male smokers also had high baseline levels of oxidised guanines in MNBC. After supplementation, only the male smokers supplemented with slow-release vitamin C tablets had increased DNA repair activity (27 (95 % CI 12, 41) % higher incision activity). These subjects also benefited from the supplementation by reduced levels of oxidised guanines in MNBC. In conclusion, nutritional status, DNA repair activity and DNA damage are linked, and beneficial effects of antioxidants might only be observed among poorly nourished subjects with high levels of oxidised DNA damage and low repair activity.

Original language	English
Journal	British Journal of Nutrition
Volume	99
Issue number	5
Pages (from-to)	1018-24
Number of pages	7
ISSN	0007-1145
DOIs	https://doi.org/10.1017/S0007114507842796
Publication status	Published - 2008

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10.1017/S0007114507842796

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title = "DNA repair phenotype and dietary antioxidant supplementation",

abstract = "Phytochemicals may protect cellular DNA by direct antioxidant effect or modulation of the DNA repair activity. We investigated the repair activity towards oxidised DNA in human mononuclear blood cells (MNBC) in two placebo-controlled antioxidant intervention studies as follows: (1) well-nourished subjects who ingested 600 g fruits and vegetables, or tablets containing the equivalent amount of vitamins and minerals, for 24 d; (2) poorly nourished male smokers who ingested 500 mg vitamin C/d as slow- or plain-release formulations together with 182 mg vitamin E/d for 4 weeks. The mean baseline levels of DNA repair incisions were 65.2 (95 % CI 60.4, 70.0) and 86.1 (95 % CI 76.2, 99.9) among the male smokers and well-nourished subjects, respectively. The male smokers also had high baseline levels of oxidised guanines in MNBC. After supplementation, only the male smokers supplemented with slow-release vitamin C tablets had increased DNA repair activity (27 (95 % CI 12, 41) % higher incision activity). These subjects also benefited from the supplementation by reduced levels of oxidised guanines in MNBC. In conclusion, nutritional status, DNA repair activity and DNA damage are linked, and beneficial effects of antioxidants might only be observed among poorly nourished subjects with high levels of oxidised DNA damage and low repair activity.",

author = "Serena Guarnieri and Steffen Loft and Patrizia Riso and Marisa Porrini and Lotte Risom and Poulsen, {Henrik Enghusen} and Dragsted, {Lars Ove} and Peter M{\o}ller",

year = "2008",

doi = "10.1017/S0007114507842796",

language = "English",

volume = "99",

pages = "1018--24",

journal = "British Journal of Nutrition",

issn = "0007-1145",

publisher = "Cambridge University Press",

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TY - JOUR

T1 - DNA repair phenotype and dietary antioxidant supplementation

AU - Guarnieri, Serena

AU - Loft, Steffen

AU - Riso, Patrizia

AU - Porrini, Marisa

AU - Risom, Lotte

AU - Poulsen, Henrik Enghusen

AU - Dragsted, Lars Ove

AU - Møller, Peter

PY - 2008

Y1 - 2008

N2 - Phytochemicals may protect cellular DNA by direct antioxidant effect or modulation of the DNA repair activity. We investigated the repair activity towards oxidised DNA in human mononuclear blood cells (MNBC) in two placebo-controlled antioxidant intervention studies as follows: (1) well-nourished subjects who ingested 600 g fruits and vegetables, or tablets containing the equivalent amount of vitamins and minerals, for 24 d; (2) poorly nourished male smokers who ingested 500 mg vitamin C/d as slow- or plain-release formulations together with 182 mg vitamin E/d for 4 weeks. The mean baseline levels of DNA repair incisions were 65.2 (95 % CI 60.4, 70.0) and 86.1 (95 % CI 76.2, 99.9) among the male smokers and well-nourished subjects, respectively. The male smokers also had high baseline levels of oxidised guanines in MNBC. After supplementation, only the male smokers supplemented with slow-release vitamin C tablets had increased DNA repair activity (27 (95 % CI 12, 41) % higher incision activity). These subjects also benefited from the supplementation by reduced levels of oxidised guanines in MNBC. In conclusion, nutritional status, DNA repair activity and DNA damage are linked, and beneficial effects of antioxidants might only be observed among poorly nourished subjects with high levels of oxidised DNA damage and low repair activity.

AB - Phytochemicals may protect cellular DNA by direct antioxidant effect or modulation of the DNA repair activity. We investigated the repair activity towards oxidised DNA in human mononuclear blood cells (MNBC) in two placebo-controlled antioxidant intervention studies as follows: (1) well-nourished subjects who ingested 600 g fruits and vegetables, or tablets containing the equivalent amount of vitamins and minerals, for 24 d; (2) poorly nourished male smokers who ingested 500 mg vitamin C/d as slow- or plain-release formulations together with 182 mg vitamin E/d for 4 weeks. The mean baseline levels of DNA repair incisions were 65.2 (95 % CI 60.4, 70.0) and 86.1 (95 % CI 76.2, 99.9) among the male smokers and well-nourished subjects, respectively. The male smokers also had high baseline levels of oxidised guanines in MNBC. After supplementation, only the male smokers supplemented with slow-release vitamin C tablets had increased DNA repair activity (27 (95 % CI 12, 41) % higher incision activity). These subjects also benefited from the supplementation by reduced levels of oxidised guanines in MNBC. In conclusion, nutritional status, DNA repair activity and DNA damage are linked, and beneficial effects of antioxidants might only be observed among poorly nourished subjects with high levels of oxidised DNA damage and low repair activity.

U2 - 10.1017/S0007114507842796

DO - 10.1017/S0007114507842796

M3 - Journal article

C2 - 17925050

SN - 0007-1145

VL - 99

SP - 1018

EP - 1024

JO - British Journal of Nutrition

JF - British Journal of Nutrition

IS - 5

ER -

DNA repair phenotype and dietary antioxidant supplementation

Abstract

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