DNA polymorphism of HLA class II genes in systemic lupus erythematosus

J B Cowland; V Andersen; P Halberg; N Morling

DNA polymorphism of HLA class II genes in systemic lupus erythematosus

J B Cowland, V Andersen, P Halberg, N Morling

Section of Forensic Genetics

16 Citations (Scopus)

Abstract

We investigated the DNA restriction fragment length polymorphism (RFLP) of the major histocompatibility complex (MHC) genes: HLA-DRB, -DQA, -DQB, -DPB in 24 Danish patients with systemic lupus erythematosus (SLE) and in 102 healthy Danes. A highly significant increase of the frequency of the DR3- and DRw6-associated 7.00 kb DRB TaqI DNA fragment was found in SLE patients compared to normal controls (83.3% vs 35.5%; RR = 9.1, p < 10(-4). The frequencies of the DQA1*0501-associated 4.56 kb DQA TaqI fragment and the DRB3*01/03-associated 9.79 kb TaqI fragment were also found to be significantly increased in SLE patients (70.8% vs 29.7%; RR = 5.8, p < 10(-2) for the DQA fragment and 70.8% vs 36.1%; RR = 4.3, p < 0.05 for the DRB3 fragment). Less extensive and insignificant increases of the frequencies of the DR3-associated DQB and DPB fragments were observed. The frequencies of the DR2-associated DRB, DQA, and DQB fragments were comparable to those found in normal controls.

Original language	English
Journal	HLA
Volume	43
Issue number	1
Pages (from-to)	34-7
Number of pages	3
ISSN	2059-2302
Publication status	Published - 1994

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@article{2225c6b0e4b811deba73000ea68e967b,

title = "DNA polymorphism of HLA class II genes in systemic lupus erythematosus",

abstract = "We investigated the DNA restriction fragment length polymorphism (RFLP) of the major histocompatibility complex (MHC) genes: HLA-DRB, -DQA, -DQB, -DPB in 24 Danish patients with systemic lupus erythematosus (SLE) and in 102 healthy Danes. A highly significant increase of the frequency of the DR3- and DRw6-associated 7.00 kb DRB TaqI DNA fragment was found in SLE patients compared to normal controls (83.3% vs 35.5%; RR = 9.1, p < 10(-4). The frequencies of the DQA1*0501-associated 4.56 kb DQA TaqI fragment and the DRB3*01/03-associated 9.79 kb TaqI fragment were also found to be significantly increased in SLE patients (70.8% vs 29.7%; RR = 5.8, p < 10(-2) for the DQA fragment and 70.8% vs 36.1%; RR = 4.3, p < 0.05 for the DRB3 fragment). Less extensive and insignificant increases of the frequencies of the DR3-associated DQB and DPB fragments were observed. The frequencies of the DR2-associated DRB, DQA, and DQB fragments were comparable to those found in normal controls.",

author = "Cowland, {J B} and V Andersen and P Halberg and N Morling",

note = "Keywords: DNA; HLA-DP Antigens; HLA-DQ Antigens; HLA-DR Antigens; Histocompatibility Antigens Class II; Humans; Lupus Erythematosus, Systemic; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length",

year = "1994",

language = "English",

volume = "43",

pages = "34--7",

journal = "HLA",

issn = "2059-2302",

publisher = "Wiley",

number = "1",

}

TY - JOUR

T1 - DNA polymorphism of HLA class II genes in systemic lupus erythematosus

AU - Cowland, J B

AU - Andersen, V

AU - Halberg, P

AU - Morling, N

N1 - Keywords: DNA; HLA-DP Antigens; HLA-DQ Antigens; HLA-DR Antigens; Histocompatibility Antigens Class II; Humans; Lupus Erythematosus, Systemic; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length

PY - 1994

Y1 - 1994

N2 - We investigated the DNA restriction fragment length polymorphism (RFLP) of the major histocompatibility complex (MHC) genes: HLA-DRB, -DQA, -DQB, -DPB in 24 Danish patients with systemic lupus erythematosus (SLE) and in 102 healthy Danes. A highly significant increase of the frequency of the DR3- and DRw6-associated 7.00 kb DRB TaqI DNA fragment was found in SLE patients compared to normal controls (83.3% vs 35.5%; RR = 9.1, p < 10(-4). The frequencies of the DQA1*0501-associated 4.56 kb DQA TaqI fragment and the DRB3*01/03-associated 9.79 kb TaqI fragment were also found to be significantly increased in SLE patients (70.8% vs 29.7%; RR = 5.8, p < 10(-2) for the DQA fragment and 70.8% vs 36.1%; RR = 4.3, p < 0.05 for the DRB3 fragment). Less extensive and insignificant increases of the frequencies of the DR3-associated DQB and DPB fragments were observed. The frequencies of the DR2-associated DRB, DQA, and DQB fragments were comparable to those found in normal controls.

AB - We investigated the DNA restriction fragment length polymorphism (RFLP) of the major histocompatibility complex (MHC) genes: HLA-DRB, -DQA, -DQB, -DPB in 24 Danish patients with systemic lupus erythematosus (SLE) and in 102 healthy Danes. A highly significant increase of the frequency of the DR3- and DRw6-associated 7.00 kb DRB TaqI DNA fragment was found in SLE patients compared to normal controls (83.3% vs 35.5%; RR = 9.1, p < 10(-4). The frequencies of the DQA1*0501-associated 4.56 kb DQA TaqI fragment and the DRB3*01/03-associated 9.79 kb TaqI fragment were also found to be significantly increased in SLE patients (70.8% vs 29.7%; RR = 5.8, p < 10(-2) for the DQA fragment and 70.8% vs 36.1%; RR = 4.3, p < 0.05 for the DRB3 fragment). Less extensive and insignificant increases of the frequencies of the DR3-associated DQB and DPB fragments were observed. The frequencies of the DR2-associated DRB, DQA, and DQB fragments were comparable to those found in normal controls.

M3 - Journal article

C2 - 7912858

SN - 2059-2302

VL - 43

SP - 34

EP - 37

JO - HLA

JF - HLA

IS - 1

ER -

DNA polymorphism of HLA class II genes in systemic lupus erythematosus

Abstract

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