DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis.

Wim J J Soppe; Zusana Jasencakova; Andreas Houben; Tetsuji Kakutani; Armin Meister; Michael S Huang; Steven E Jacobsen; Ingo Schubert; Paul F Fransz

DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis.

Wim J J Soppe, Zusana Jasencakova, Andreas Houben, Tetsuji Kakutani, Armin Meister, Michael S Huang, Steven E Jacobsen, Ingo Schubert, Paul F Fransz

369 Citations (Scopus)

Abstract

We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F(1) hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.

Original language	English
Journal	EMBO Journal
Volume	21
Issue number	23
Pages (from-to)	6549-59
Number of pages	10
ISSN	0261-4189
Publication status	Published - 2002
Externally published	Yes

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title = "DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis.",

abstract = "We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F(1) hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.",

author = "Soppe, {Wim J J} and Zusana Jasencakova and Andreas Houben and Tetsuji Kakutani and Armin Meister and Huang, {Michael S} and Jacobsen, {Steven E} and Ingo Schubert and Fransz, {Paul F}",

note = "Keywords: Arabidopsis; Arabidopsis Proteins; DNA Methylation; DNA-Binding Proteins; Gene Silencing; Heterochromatin; Histones; Mutation; Transcription Factors",

year = "2002",

language = "English",

volume = "21",

pages = "6549--59",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell",

number = "23",

}

TY - JOUR

T1 - DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis.

AU - Soppe, Wim J J

AU - Jasencakova, Zusana

AU - Houben, Andreas

AU - Kakutani, Tetsuji

AU - Meister, Armin

AU - Huang, Michael S

AU - Jacobsen, Steven E

AU - Schubert, Ingo

AU - Fransz, Paul F

N1 - Keywords: Arabidopsis; Arabidopsis Proteins; DNA Methylation; DNA-Binding Proteins; Gene Silencing; Heterochromatin; Histones; Mutation; Transcription Factors

PY - 2002

Y1 - 2002

N2 - We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F(1) hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.

AB - We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F(1) hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.

M3 - Journal article

C2 - 12456661

SN - 0261-4189

VL - 21

SP - 6549

EP - 6559

JO - EMBO Journal

JF - EMBO Journal

IS - 23

ER -

DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis.

Abstract

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