Distribution of RAPD Markers on a Linkage Map of Barley

H. Giese; A. G. Holm‐Jensen; H. Mathiassen; B. Kjær; S. K. Rasmussen; H. Bay; J. Jensen

doi:10.1111/j.1601-5223.1994.00267.x

Distribution of RAPD Markers on a Linkage Map of Barley

H. Giese^*, A. G. Holm‐Jensen, H. Mathiassen, B. Kjær, S. K. Rasmussen, H. Bay, J. Jensen

^*Corresponding author for this work

49 Citations (Scopus)

Abstract

The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.

Original language	English
Journal	Hereditas
Volume	120
Issue number	3
Pages (from-to)	267-273
Number of pages	7
ISSN	0018-0661
DOIs	https://doi.org/10.1111/j.1601-5223.1994.00267.x
Publication status	Published - 1 Jan 1994

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title = "Distribution of RAPD Markers on a Linkage Map of Barley",

abstract = "The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.",

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T1 - Distribution of RAPD Markers on a Linkage Map of Barley

AU - Giese, H.

AU - Holm‐Jensen, A. G.

AU - Mathiassen, H.

AU - Kjær, B.

AU - Rasmussen, S. K.

AU - Bay, H.

AU - Jensen, J.

PY - 1994/1/1

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N2 - The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.

AB - The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.

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Distribution of RAPD Markers on a Linkage Map of Barley

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