Distribution of RAPD Markers on a Linkage Map of Barley

H. Giese; A. G. Holm‐Jensen; H. Mathiassen; B. Kjær; S. K. Rasmussen; H. Bay; J. Jensen

doi:10.1111/j.1601-5223.1994.00267.x

Distribution of RAPD Markers on a Linkage Map of Barley

H. Giese^*, A. G. Holm‐Jensen, H. Mathiassen, B. Kjær, S. K. Rasmussen, H. Bay, J. Jensen

^*Corresponding author af dette arbejde

49 Citationer (Scopus)

Abstract

The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.

Originalsprog	Engelsk
Tidsskrift	Hereditas
Vol/bind	120
Udgave nummer	3
Sider (fra-til)	267-273
Antal sider	7
ISSN	0018-0661
DOI	https://doi.org/10.1111/j.1601-5223.1994.00267.x
Status	Udgivet - 1 jan. 1994

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AU - Giese, H.

AU - Holm‐Jensen, A. G.

AU - Mathiassen, H.

AU - Kjær, B.

AU - Rasmussen, S. K.

AU - Bay, H.

AU - Jensen, J.

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AB - The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.

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Distribution of RAPD Markers on a Linkage Map of Barley

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