Distinct in vitro interaction pattern of dopamine receptor subtypes with adaptor proteins involved in post-endocytotic receptor targeting

TY - JOUR

T1 - Distinct in vitro interaction pattern of dopamine receptor subtypes with adaptor proteins involved in post-endocytotic receptor targeting

AU - Heydorn, Arne

AU - Søndergaard, Birgitte P

AU - Hadrup, Niels

AU - Holst, Birgitte

AU - Haft, Carol Renfrew

AU - Schwartz, Thue W

N1 - Keywords: Animals; Carrier Proteins; Endocytosis; Escherichia coli; Glutathione Transferase; Humans; N-Ethylmaleimide-Sensitive Proteins; Phosphoproteins; Protein Isoforms; Rats; Receptors, Dopamine; Recombinant Fusion Proteins; Signal Transduction; Sodium-Hydrogen Antiporter; Sulfur Radioisotopes; Vesicular Transport Proteins

PY - 2004

Y1 - 2004

N2 - The mechanisms underlying targeted sorting of endocytosed receptors for recycling to the plasma membrane or degradation in lysosomes are poorly understood. In this report, the C-terminal tails of the five dopamine receptors (D1-D5) were expressed as glutathione S-transferase (GST) fusion proteins and studied for their interaction with ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) and N-ethylmaleimide-sensitive factor (NSF), which are known to be involved in post-endocytic recycling of receptors back to the plasma membrane, and with sorting nexin 1 (SNX1), known to be involved in targeting receptors to lysosomal degradation. EBP50 did not bind any of the dopamine receptor tails. NSF bound strongly to D1 and D5 and only weakly to D2, D3 and D4. However, SNX1 clearly distinguished between D1 and D5, as only D5 bound strongly to this protein. This report shows that there are distinct interaction patterns for NSF and SNX1 to the various dopamine receptor subtypes.

AB - The mechanisms underlying targeted sorting of endocytosed receptors for recycling to the plasma membrane or degradation in lysosomes are poorly understood. In this report, the C-terminal tails of the five dopamine receptors (D1-D5) were expressed as glutathione S-transferase (GST) fusion proteins and studied for their interaction with ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) and N-ethylmaleimide-sensitive factor (NSF), which are known to be involved in post-endocytic recycling of receptors back to the plasma membrane, and with sorting nexin 1 (SNX1), known to be involved in targeting receptors to lysosomal degradation. EBP50 did not bind any of the dopamine receptor tails. NSF bound strongly to D1 and D5 and only weakly to D2, D3 and D4. However, SNX1 clearly distinguished between D1 and D5, as only D5 bound strongly to this protein. This report shows that there are distinct interaction patterns for NSF and SNX1 to the various dopamine receptor subtypes.

M3 - Journal article

C2 - 14706863

SN - 0014-5793

VL - 556

SP - 276

EP - 280

JO - F E B S Letters

JF - F E B S Letters

IS - 1-3

ER -