Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Yding Wolff; Steffen Eickhardt; Maria K Björnsdottir; Claus Moser; Thomas Bjarnsholt; Niels Høiby; Trine R Thomsen

doi:10.1128/JCM.00190-13

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Yding Wolff, Steffen Eickhardt, Maria K Björnsdottir, Claus Moser, Thomas Bjarnsholt, Niels Høiby, Trine R Thomsen

5 Citations (Scopus)

Abstract

In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	4
Pages (from-to)	1281-4
Number of pages	4
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.00190-13
Publication status	Published - Apr 2013

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title = "Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections",

abstract = "In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.",

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AU - Wolff, Tine Yding

AU - Eickhardt, Steffen

AU - Björnsdottir, Maria K

AU - Moser, Claus

AU - Bjarnsholt, Thomas

AU - Høiby, Niels

AU - Thomsen, Trine R

PY - 2013/4

Y1 - 2013/4

N2 - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

AB - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

U2 - 10.1128/JCM.00190-13

DO - 10.1128/JCM.00190-13

M3 - Journal article

C2 - 23363825

SN - 0095-1137

VL - 51

SP - 1281

EP - 1284

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 4

ER -

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Abstract

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