Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Yding Wolff; Steffen Eickhardt; Maria K Björnsdottir; Claus Moser; Thomas Bjarnsholt; Niels Høiby; Trine R Thomsen

doi:10.1128/JCM.00190-13

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Yding Wolff, Steffen Eickhardt, Maria K Björnsdottir, Claus Moser, Thomas Bjarnsholt, Niels Høiby, Trine R Thomsen

5 Citationer (Scopus)

Abstract

In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

Originalsprog	Engelsk
Tidsskrift	Journal of Clinical Microbiology
Vol/bind	51
Udgave nummer	4
Sider (fra-til)	1281-4
Antal sider	4
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.00190-13
Status	Udgivet - apr. 2013

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10.1128/JCM.00190-13

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title = "Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections",

abstract = "In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.",

author = "Wolff, {Tine Yding} and Steffen Eickhardt and Bj{\"o}rnsdottir, {Maria K} and Claus Moser and Thomas Bjarnsholt and Niels H{\o}iby and Thomsen, {Trine R}",

year = "2013",

month = apr,

doi = "10.1128/JCM.00190-13",

language = "English",

volume = "51",

pages = "1281--4",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

AU - Wolff, Tine Yding

AU - Eickhardt, Steffen

AU - Björnsdottir, Maria K

AU - Moser, Claus

AU - Bjarnsholt, Thomas

AU - Høiby, Niels

AU - Thomsen, Trine R

PY - 2013/4

Y1 - 2013/4

N2 - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

AB - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

U2 - 10.1128/JCM.00190-13

DO - 10.1128/JCM.00190-13

M3 - Journal article

C2 - 23363825

SN - 0095-1137

VL - 51

SP - 1281

EP - 1284

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 4

ER -

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Abstract

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