Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

Ivana Domljanovic; Elisabeth Rexen Ulven; Trond Ulven; Rasmus P. Thomsen; Anders H. Okholm; Jørgen Kjems; Anne Voss; Maria Taskova; Kira Astakhova

doi:10.1021/acsomega.8b00424

Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

Ivana Domljanovic, Elisabeth Rexen Ulven, Trond Ulven, Rasmus P. Thomsen, Anders H. Okholm, Jørgen Kjems, Anne Voss, Maria Taskova, Kira Astakhova^*

^*Corresponding author for this work

Department of Drug Design and Pharmacology

4 Citations (Scopus)

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Abstract

Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.

Original language	English
Journal	ACS Omega
Volume	3
Issue number	7
Pages (from-to)	7580-7586
Number of pages	7
ISSN	2470-1343
DOIs	https://doi.org/10.1021/acsomega.8b00424
Publication status	Published - 31 Jul 2018

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title = "Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum",

abstract = "Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.",

author = "Ivana Domljanovic and {Rexen Ulven}, Elisabeth and Trond Ulven and Thomsen, {Rasmus P.} and Okholm, {Anders H.} and J{\o}rgen Kjems and Anne Voss and Maria Taskova and Kira Astakhova",

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T1 - Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

AU - Domljanovic, Ivana

AU - Rexen Ulven, Elisabeth

AU - Ulven, Trond

AU - Thomsen, Rasmus P.

AU - Okholm, Anders H.

AU - Kjems, Jørgen

AU - Voss, Anne

AU - Taskova, Maria

AU - Astakhova, Kira

PY - 2018/7/31

Y1 - 2018/7/31

N2 - Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.

AB - Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.

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Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

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