Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

TY - JOUR

T1 - Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

AU - Domljanovic, Ivana

AU - Rexen Ulven, Elisabeth

AU - Ulven, Trond

AU - Thomsen, Rasmus P.

AU - Okholm, Anders H.

AU - Kjems, Jørgen

AU - Voss, Anne

AU - Taskova, Maria

AU - Astakhova, Kira

PY - 2018/7/31

Y1 - 2018/7/31

N2 - Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.

AB - Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.

U2 - 10.1021/acsomega.8b00424

DO - 10.1021/acsomega.8b00424

M3 - Journal article

AN - SCOPUS:85049784084

SN - 2470-1343

VL - 3

SP - 7580

EP - 7586

JO - ACS Omega

JF - ACS Omega

IS - 7

ER -