Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

Michaela Rothová; Jurriaan J Hölzenspies; Alessandra Livigni; Santiago Nahuel Villegas; Joshua M Brickman

doi:10.1002/9780470151808.sc01g03s36

Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

Michaela Rothová, Jurriaan J Hölzenspies, Alessandra Livigni, Santiago Nahuel Villegas, Joshua M Brickman

Stem Cell and developmental Biology - early embryonic lineage specification Lab

1 Citation (Scopus)

Abstract

Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.

Original language	English
Journal	Current Protocols in Stem Cell Biology
Volume	36
Pages (from-to)	1G.3.1-12
ISSN	1938-8969
DOIs	https://doi.org/10.1002/9780470151808.sc01g03s36
Publication status	Published - 3 Feb 2016

Keywords

Journal Article
Research Support, Non-U.S. Gov't

Access to Document

10.1002/9780470151808.sc01g03s36

Cite this

@article{c2d483d31e8a437d84665dd78621ca21,

title = "Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors",

abstract = "Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.",

keywords = "Journal Article, Research Support, Non-U.S. Gov't",

author = "Michaela Rothov{\'a} and H{\"o}lzenspies, {Jurriaan J} and Alessandra Livigni and Villegas, {Santiago Nahuel} and Brickman, {Joshua M}",

note = "Copyright {\textcopyright} 2016 John Wiley & Sons, Inc.",

year = "2016",

month = feb,

day = "3",

doi = "10.1002/9780470151808.sc01g03s36",

language = "English",

volume = "36",

pages = "1G.3.1--12",

journal = "Current Protocols in Stem Cell Biology",

issn = "1938-8969",

publisher = "JohnWiley & Sons, Inc.",

}

TY - JOUR

T1 - Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

AU - Rothová, Michaela

AU - Hölzenspies, Jurriaan J

AU - Livigni, Alessandra

AU - Villegas, Santiago Nahuel

AU - Brickman, Joshua M

PY - 2016/2/3

Y1 - 2016/2/3

N2 - Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.

AB - Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/9780470151808.sc01g03s36

DO - 10.1002/9780470151808.sc01g03s36

M3 - Journal article

C2 - 26840225

SN - 1938-8969

VL - 36

SP - 1G.3.1-12

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

ER -

Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

Abstract

Keywords

Access to Document

Fingerprint

Cite this