Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

TY - JOUR

T1 - Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

AU - Rothová, Michaela

AU - Hölzenspies, Jurriaan J

AU - Livigni, Alessandra

AU - Villegas, Santiago Nahuel

AU - Brickman, Joshua M

N1 - Copyright © 2016 John Wiley & Sons, Inc.

PY - 2016/2/3

Y1 - 2016/2/3

N2 - Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.

AB - Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/9780470151808.sc01g03s36

DO - 10.1002/9780470151808.sc01g03s36

M3 - Journal article

C2 - 26840225

SN - 1938-8969

VL - 36

SP - 1G.3.1-12

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

ER -