Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

Janina D'Alvise; Rasmus Mortensen; Steen H Hansen; Christian Janfelt

doi:10.1007/s00216-014-7802-z

Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

Janina D'Alvise, Rasmus Mortensen, Steen H Hansen, Christian Janfelt

23 Citations (Scopus)

Abstract

Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies. [Figure not available: see fulltext.]

Original language	English
Journal	Analytical and Bioanalytical Chemistry
Volume	406
Issue number	15
Pages (from-to)	3735-42
Number of pages	8
ISSN	1618-2642
DOIs	https://doi.org/10.1007/s00216-014-7802-z
Publication status	Published - Jun 2014

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title = "Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging",

abstract = "Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies. [Figure not available: see fulltext.]",

author = "Janina D'Alvise and Rasmus Mortensen and Hansen, {Steen H} and Christian Janfelt",

year = "2014",

month = jun,

doi = "10.1007/s00216-014-7802-z",

language = "English",

volume = "406",

pages = "3735--42",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer",

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TY - JOUR

T1 - Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

AU - D'Alvise, Janina

AU - Mortensen, Rasmus

AU - Hansen, Steen H

AU - Janfelt, Christian

PY - 2014/6

Y1 - 2014/6

N2 - Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies. [Figure not available: see fulltext.]

AB - Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies. [Figure not available: see fulltext.]

U2 - 10.1007/s00216-014-7802-z

DO - 10.1007/s00216-014-7802-z

M3 - Journal article

C2 - 24722877

SN - 1618-2642

VL - 406

SP - 3735

EP - 3742

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 15

ER -

Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

Abstract

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