Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs

B O Lindhardt; Ib Christian Bygbjerg; K Ulrich; H D Petersen; I Lausen; B Frederiksen

Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs

B O Lindhardt, Ib Christian Bygbjerg, K Ulrich, H D Petersen, I Lausen, B Frederiksen

21 Citations (Scopus)

Abstract

A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.

Original language	English
Journal	Journal of Virological Methods
Volume	18
Issue number	1
Pages (from-to)	73-7
Number of pages	5
ISSN	0166-0934
Publication status	Published - 1 Oct 1987

Keywords

Africa, Central
Africa, Eastern
Antibodies, Viral
Denmark
Enzyme-Linked Immunosorbent Assay
HIV
HIV Antibodies
HIV Seropositivity
Humans
Paper

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs",

abstract = "A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.",

keywords = "Africa, Central, Africa, Eastern, Antibodies, Viral, Denmark, Enzyme-Linked Immunosorbent Assay, HIV, HIV Antibodies, HIV Seropositivity, Humans, Paper",

author = "Lindhardt, {B O} and Bygbjerg, {Ib Christian} and K Ulrich and Petersen, {H D} and I Lausen and B Frederiksen",

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T1 - Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs

AU - Lindhardt, B O

AU - Bygbjerg, Ib Christian

AU - Ulrich, K

AU - Petersen, H D

AU - Lausen, I

AU - Frederiksen, B

PY - 1987/10/1

Y1 - 1987/10/1

N2 - A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.

AB - A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.

KW - Africa, Central

KW - Africa, Eastern

KW - Antibodies, Viral

KW - Denmark

KW - Enzyme-Linked Immunosorbent Assay

KW - HIV

KW - HIV Antibodies

KW - HIV Seropositivity

KW - Humans

KW - Paper

M3 - Journal article

C2 - 3480292

SN - 0166-0934

VL - 18

SP - 73

EP - 77

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs

Abstract

Keywords

UN SDGs

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