Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs

TY - JOUR

T1 - Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs

AU - Lindhardt, B O

AU - Bygbjerg, Ib Christian

AU - Ulrich, K

AU - Petersen, H D

AU - Lausen, I

AU - Frederiksen, B

PY - 1987/10/1

Y1 - 1987/10/1

N2 - A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.

AB - A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.

KW - Africa, Central

KW - Africa, Eastern

KW - Antibodies, Viral

KW - Denmark

KW - Enzyme-Linked Immunosorbent Assay

KW - HIV

KW - HIV Antibodies

KW - HIV Seropositivity

KW - Humans

KW - Paper

M3 - Journal article

C2 - 3480292

SN - 0166-0934

VL - 18

SP - 73

EP - 77

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -