Design and validation of conditional ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05

TY - JOUR

T1 - Design and validation of conditional ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05

AU - Frøsig, Thomas Mørch

AU - Yap, Jiawei

AU - Seremet, Tina

AU - Lyngaa, Rikke

AU - Svane, Inge Marie

AU - Thor Straten, Per

AU - Heemskerk, Mirjam H M

AU - Grotenbreg, Gijsbert M

AU - Hadrup, Sine Reker

N1 - © 2015 International Society for Advancement of Cytometry.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

AB - We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

U2 - 10.1002/cyto.a.22689

DO - 10.1002/cyto.a.22689

M3 - Journal article

C2 - 26033882

SN - 1046-7386

VL - 87

SP - 967

EP - 975

JO - Cytometry. Supplement : the journal of the Society for Analytical Cytology

JF - Cytometry. Supplement : the journal of the Society for Analytical Cytology

IS - 10

ER -