Design and application of an optical sensor for simultaneous imaging of pH and dissolved O2 with low cross-talk

Maria Mosshammer; Martin Strobl; Michael Kühl; Ingo Klimant; Sergey M. Borisov; Klaus Koren

doi:10.1021/acssensors.6b00071

Design and application of an optical sensor for simultaneous imaging of pH and dissolved O₂ with low cross-talk

Maria Mosshammer, Martin Strobl, Michael Kühl, Ingo Klimant, Sergey M. Borisov, Klaus Koren

Marine Biology

41 Citations (Scopus)

131 Downloads (Pure)

Abstract

Visualization and quantification of analytes such as O₂ or pH is essential in biological research. Here we present the design and application of a new optical dual-analyte sensor for imaging, optimized to have low cross-sensitivity between the two analytes O₂ and pH. The used indicator and reference dyes were selected to match the different channels of a commercial 2CCD (RGB + NIR) camera. A red-light emitting O₂-sensitive europium complex (Eu(HPhN)₃dpp) with a dynamic range of 0-20% O₂ in the finished sensor was combined with a near-infrared emitting pH indicator (OHButoxy-aza-BODIPY) with a dynamic range of pH 7.2-8.8. To enable ratiometric readout, an inert reference coumarin dye (Bu₃Coum) was co-immobilized with the optical indicators. In order to maximize the sensor signal, inert diamond powder was added to one sensor layer as a simple way to increase scattering of light within the sensor. Furthermore, the addition of an optical isolation layer enabled measurements in highly fluorescent samples, such as algal biofilms. The sensor was tested in a marine photosynthetic microbial mat.

Original language	English
Journal	ACS Sensors
Volume	1
Issue number	6
Pages (from-to)	681-687
Number of pages	7
ISSN	2379-3694
DOIs	https://doi.org/10.1021/acssensors.6b00071
Publication status	Published - 24 Jun 2016

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Cite this

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title = "Design and application of an optical sensor for simultaneous imaging of pH and dissolved O2 with low cross-talk",

abstract = "Visualization and quantification of analytes such as O2 or pH is essential in biological research. Here we present the design and application of a new optical dual-analyte sensor for imaging, optimized to have low cross-sensitivity between the two analytes O2 and pH. The used indicator and reference dyes were selected to match the different channels of a commercial 2CCD (RGB + NIR) camera. A red-light emitting O2-sensitive europium complex (Eu(HPhN)3dpp) with a dynamic range of 0-20% O2 in the finished sensor was combined with a near-infrared emitting pH indicator (OHButoxy-aza-BODIPY) with a dynamic range of pH 7.2-8.8. To enable ratiometric readout, an inert reference coumarin dye (Bu3Coum) was co-immobilized with the optical indicators. In order to maximize the sensor signal, inert diamond powder was added to one sensor layer as a simple way to increase scattering of light within the sensor. Furthermore, the addition of an optical isolation layer enabled measurements in highly fluorescent samples, such as algal biofilms. The sensor was tested in a marine photosynthetic microbial mat.",

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AU - Mosshammer, Maria

AU - Strobl, Martin

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AU - Borisov, Sergey M.

AU - Koren, Klaus

PY - 2016/6/24

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N2 - Visualization and quantification of analytes such as O2 or pH is essential in biological research. Here we present the design and application of a new optical dual-analyte sensor for imaging, optimized to have low cross-sensitivity between the two analytes O2 and pH. The used indicator and reference dyes were selected to match the different channels of a commercial 2CCD (RGB + NIR) camera. A red-light emitting O2-sensitive europium complex (Eu(HPhN)3dpp) with a dynamic range of 0-20% O2 in the finished sensor was combined with a near-infrared emitting pH indicator (OHButoxy-aza-BODIPY) with a dynamic range of pH 7.2-8.8. To enable ratiometric readout, an inert reference coumarin dye (Bu3Coum) was co-immobilized with the optical indicators. In order to maximize the sensor signal, inert diamond powder was added to one sensor layer as a simple way to increase scattering of light within the sensor. Furthermore, the addition of an optical isolation layer enabled measurements in highly fluorescent samples, such as algal biofilms. The sensor was tested in a marine photosynthetic microbial mat.

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