Design and application of an optical sensor for simultaneous imaging of pH and dissolved O₂ with low cross-talk

TY - JOUR

T1 - Design and application of an optical sensor for simultaneous imaging of pH and dissolved O2 with low cross-talk

AU - Mosshammer, Maria

AU - Strobl, Martin

AU - Kühl, Michael

AU - Klimant, Ingo

AU - Borisov, Sergey M.

AU - Koren, Klaus

PY - 2016/6/24

Y1 - 2016/6/24

N2 - Visualization and quantification of analytes such as O2 or pH is essential in biological research. Here we present the design and application of a new optical dual-analyte sensor for imaging, optimized to have low cross-sensitivity between the two analytes O2 and pH. The used indicator and reference dyes were selected to match the different channels of a commercial 2CCD (RGB + NIR) camera. A red-light emitting O2-sensitive europium complex (Eu(HPhN)3dpp) with a dynamic range of 0-20% O2 in the finished sensor was combined with a near-infrared emitting pH indicator (OHButoxy-aza-BODIPY) with a dynamic range of pH 7.2-8.8. To enable ratiometric readout, an inert reference coumarin dye (Bu3Coum) was co-immobilized with the optical indicators. In order to maximize the sensor signal, inert diamond powder was added to one sensor layer as a simple way to increase scattering of light within the sensor. Furthermore, the addition of an optical isolation layer enabled measurements in highly fluorescent samples, such as algal biofilms. The sensor was tested in a marine photosynthetic microbial mat.

AB - Visualization and quantification of analytes such as O2 or pH is essential in biological research. Here we present the design and application of a new optical dual-analyte sensor for imaging, optimized to have low cross-sensitivity between the two analytes O2 and pH. The used indicator and reference dyes were selected to match the different channels of a commercial 2CCD (RGB + NIR) camera. A red-light emitting O2-sensitive europium complex (Eu(HPhN)3dpp) with a dynamic range of 0-20% O2 in the finished sensor was combined with a near-infrared emitting pH indicator (OHButoxy-aza-BODIPY) with a dynamic range of pH 7.2-8.8. To enable ratiometric readout, an inert reference coumarin dye (Bu3Coum) was co-immobilized with the optical indicators. In order to maximize the sensor signal, inert diamond powder was added to one sensor layer as a simple way to increase scattering of light within the sensor. Furthermore, the addition of an optical isolation layer enabled measurements in highly fluorescent samples, such as algal biofilms. The sensor was tested in a marine photosynthetic microbial mat.

U2 - 10.1021/acssensors.6b00071

DO - 10.1021/acssensors.6b00071

M3 - Journal article

SN - 2379-3694

VL - 1

SP - 681

EP - 687

JO - ACS Sensors

JF - ACS Sensors

IS - 6

ER -