Decoding signalling networks by mass spectrometry-based proteomics

Chuna Ram Choudhary; Matthias Mann

doi:10.1038/nrm2900

Decoding signalling networks by mass spectrometry-based proteomics

Chuna Ram Choudhary, Matthias Mann

Novo Nordisk Foundation Center for Protein Research

446 Citations (Scopus)

Abstract

Signalling networks regulate essentially all of the biology of cells and organisms in normal and disease states. Signalling is often studied using antibody-based techniques such as western blots. Large-scale 'precision proteomics' based on mass spectrometry now enables the system-wide characterization of signalling events at the levels of post-translational modifications, proteing-protein interactions and changes in protein expression. This technology delivers accurate and unbiased information about the quantitative changes of thousands of proteins and their modifications in response to any perturbation. Current studies focus on phosphorylation, but acetylation, methylation, glycosylation and ubiquitylation are also becoming amenable to investigation. Large-scale proteomics-based signalling research will fundamentally change our understanding of signalling networks.

Original language	English
Journal	Nature Reviews. Molecular Cell Biology
Volume	11
Issue number	6
Pages (from-to)	427-39
Number of pages	13
ISSN	1471-0072
DOIs	https://doi.org/10.1038/nrm2900
Publication status	Published - 1 Jun 2010

Keywords

Animals
Humans
Mass Spectrometry
Protein Processing, Post-Translational
Proteome
Proteomics
Signal Transduction

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10.1038/nrm2900

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KW - Animals

KW - Humans

KW - Mass Spectrometry

KW - Protein Processing, Post-Translational

KW - Proteome

KW - Proteomics

KW - Signal Transduction

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JO - Nature Reviews. Molecular Cell Biology

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Decoding signalling networks by mass spectrometry-based proteomics

Abstract

Keywords

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