Decoding signalling networks by mass spectrometry-based proteomics

TY - JOUR

T1 - Decoding signalling networks by mass spectrometry-based proteomics

AU - Choudhary, Chuna Ram

AU - Mann, Matthias

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Signalling networks regulate essentially all of the biology of cells and organisms in normal and disease states. Signalling is often studied using antibody-based techniques such as western blots. Large-scale 'precision proteomics' based on mass spectrometry now enables the system-wide characterization of signalling events at the levels of post-translational modifications, proteing-protein interactions and changes in protein expression. This technology delivers accurate and unbiased information about the quantitative changes of thousands of proteins and their modifications in response to any perturbation. Current studies focus on phosphorylation, but acetylation, methylation, glycosylation and ubiquitylation are also becoming amenable to investigation. Large-scale proteomics-based signalling research will fundamentally change our understanding of signalling networks.

AB - Signalling networks regulate essentially all of the biology of cells and organisms in normal and disease states. Signalling is often studied using antibody-based techniques such as western blots. Large-scale 'precision proteomics' based on mass spectrometry now enables the system-wide characterization of signalling events at the levels of post-translational modifications, proteing-protein interactions and changes in protein expression. This technology delivers accurate and unbiased information about the quantitative changes of thousands of proteins and their modifications in response to any perturbation. Current studies focus on phosphorylation, but acetylation, methylation, glycosylation and ubiquitylation are also becoming amenable to investigation. Large-scale proteomics-based signalling research will fundamentally change our understanding of signalling networks.

KW - Animals

KW - Humans

KW - Mass Spectrometry

KW - Protein Processing, Post-Translational

KW - Proteome

KW - Proteomics

KW - Signal Transduction

U2 - 10.1038/nrm2900

DO - 10.1038/nrm2900

M3 - Journal article

C2 - 20461098

SN - 1471-0072

VL - 11

SP - 427

EP - 439

JO - Nature Reviews. Molecular Cell Biology

JF - Nature Reviews. Molecular Cell Biology

IS - 6

ER -