Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?

Hanne Cecilie Thomsen; Ulf Dennis Eriksson; Inge Skrumsager Møller; Jan Kofod Schjørring

doi:10.1016/j.tplants.2014.06.002

Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?

Hanne Cecilie Thomsen, Ulf Dennis Eriksson, Inge Skrumsager Møller, Jan Kofod Schjørring^*

^*Corresponding author for this work

Section for Plant and Soil Sciences

111 Citations (Scopus)

Abstract

Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.

Original language	English
Journal	Trends in Plant Science
Volume	19
Issue number	10
Pages (from-to)	656-663
Number of pages	8
ISSN	1360-1385
DOIs	https://doi.org/10.1016/j.tplants.2014.06.002
Publication status	Published - 2014

Keywords

Glutamine synthetase
Nitrogen
Nitrogen use efficiency (NUE)
Overexpression
Regulation

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10.1016/j.tplants.2014.06.002

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title = "Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?",

abstract = "Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.",

keywords = "Glutamine synthetase, Nitrogen, Nitrogen use efficiency (NUE), Overexpression, Regulation",

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year = "2014",

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language = "English",

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T1 - Cytosolic glutamine synthetase

T2 - a target for improvement of crop nitrogen use efficiency?

AU - Thomsen, Hanne Cecilie

AU - Eriksson, Ulf Dennis

AU - Møller, Inge Skrumsager

AU - Schjørring, Jan Kofod

PY - 2014

Y1 - 2014

N2 - Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.

AB - Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.

KW - Glutamine synthetase

KW - Nitrogen

KW - Nitrogen use efficiency (NUE)

KW - Overexpression

KW - Regulation

U2 - 10.1016/j.tplants.2014.06.002

DO - 10.1016/j.tplants.2014.06.002

M3 - Journal article

C2 - 25017701

AN - SCOPUS:84908174442

SN - 1360-1385

VL - 19

SP - 656

EP - 663

JO - Trends in Plant Science

JF - Trends in Plant Science

IS - 10

ER -

Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?

Abstract

Keywords

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