Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?

Hanne Cecilie Thomsen; Ulf Dennis Eriksson; Inge Skrumsager Møller; Jan Kofod Schjørring

doi:10.1016/j.tplants.2014.06.002

Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?

Hanne Cecilie Thomsen, Ulf Dennis Eriksson, Inge Skrumsager Møller, Jan Kofod Schjørring^*

^*Corresponding author af dette arbejde

Plante- og Jordvidenskab

111 Citationer (Scopus)

Abstract

Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.

Originalsprog	Engelsk
Tidsskrift	Trends in Plant Science
Vol/bind	19
Udgave nummer	10
Sider (fra-til)	656-663
Antal sider	8
ISSN	1360-1385
DOI	https://doi.org/10.1016/j.tplants.2014.06.002
Status	Udgivet - 2014

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10.1016/j.tplants.2014.06.002

Citationsformater

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title = "Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?",

abstract = "Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.",

keywords = "Glutamine synthetase, Nitrogen, Nitrogen use efficiency (NUE), Overexpression, Regulation",

author = "Thomsen, {Hanne Cecilie} and Eriksson, {Ulf Dennis} and M{\o}ller, {Inge Skrumsager} and Schj{\o}rring, {Jan Kofod}",

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T1 - Cytosolic glutamine synthetase

T2 - a target for improvement of crop nitrogen use efficiency?

AU - Thomsen, Hanne Cecilie

AU - Eriksson, Ulf Dennis

AU - Møller, Inge Skrumsager

AU - Schjørring, Jan Kofod

PY - 2014

Y1 - 2014

N2 - Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.

AB - Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.

KW - Glutamine synthetase

KW - Nitrogen

KW - Nitrogen use efficiency (NUE)

KW - Overexpression

KW - Regulation

U2 - 10.1016/j.tplants.2014.06.002

DO - 10.1016/j.tplants.2014.06.002

M3 - Journal article

C2 - 25017701

AN - SCOPUS:84908174442

SN - 1360-1385

VL - 19

SP - 656

EP - 663

JO - Trends in Plant Science

JF - Trends in Plant Science

IS - 10

ER -

Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?

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