Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Karin Holmfeldt; Dusko Odic; Matthew B. Sullivan; Mathias Middelboe; Lasse Riemann

doi:10.1128/aem.06580-11

Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Karin Holmfeldt, Dusko Odic, Matthew B. Sullivan, Mathias Middelboe, Lasse Riemann

Marine Biology

40 Citations (Scopus)

Abstract

This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	3
Pages (from-to)	892-894
ISSN	0099-2240
DOIs	https://doi.org/10.1128/aem.06580-11
Publication status	Published - Feb 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1128/aem.06580-11

Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stainsFinal published version, 559 KB

https://aem.asm.org/content/aem/78/3/892.full.pdf

Cite this

@article{78897c23477b4ac8933cf0ea0cab03fd,

title = "Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains",

abstract = "This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.",

author = "Karin Holmfeldt and Dusko Odic and Sullivan, {Matthew B.} and Mathias Middelboe and Lasse Riemann",

year = "2012",

month = feb,

doi = "10.1128/aem.06580-11",

language = "English",

volume = "78",

pages = "892--894",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "3",

}

TY - JOUR

T1 - Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

AU - Holmfeldt, Karin

AU - Odic, Dusko

AU - Sullivan, Matthew B.

AU - Middelboe, Mathias

AU - Riemann, Lasse

PY - 2012/2

Y1 - 2012/2

N2 - This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

AB - This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

U2 - 10.1128/aem.06580-11

DO - 10.1128/aem.06580-11

M3 - Journal article

SN - 0099-2240

VL - 78

SP - 892

EP - 894

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 3

ER -

Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this