Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Karin Holmfeldt; Dusko Odic; Matthew B. Sullivan; Mathias Middelboe; Lasse Riemann

doi:10.1128/aem.06580-11

Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Karin Holmfeldt, Dusko Odic, Matthew B. Sullivan, Mathias Middelboe, Lasse Riemann

Marine Biological Section

40 Citationer (Scopus)

Abstract

This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

Originalsprog	Engelsk
Tidsskrift	Applied and Environmental Microbiology
Vol/bind	78
Udgave nummer	3
Sider (fra-til)	892-894
ISSN	0099-2240
DOI	https://doi.org/10.1128/aem.06580-11
Status	Udgivet - feb. 2012

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10.1128/aem.06580-11

Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stainsForlagets udgivne version, 559 KB

https://aem.asm.org/content/aem/78/3/892.full.pdf

Citationsformater

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abstract = "This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.",

author = "Karin Holmfeldt and Dusko Odic and Sullivan, {Matthew B.} and Mathias Middelboe and Lasse Riemann",

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AU - Middelboe, Mathias

AU - Riemann, Lasse

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AB - This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

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DO - 10.1128/aem.06580-11

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Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Abstract

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