Crystallization of mutant forms of glutaredoxin Grx1p from yeast

Kjell Ove Håkansson; Henrik Østergaard; Jakob R. Winther

doi:10.1107/S1744309106031216

Crystallization of mutant forms of glutaredoxin Grx1p from yeast

Kjell Ove Håkansson, Henrik Østergaard, Jakob R. Winther

2 Citations (Scopus)

Abstract

Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 Å (synchrotron radiation) and 2.7 Å (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO₄ which diffract synchrotron radiation to 2.7 Å.

Original language	English
Journal	Acta Crystallographica. Section F : Structural Biology and Crystallization Communications
Volume	62
Issue number	9
Pages (from-to)	920-922
ISSN	1744-3091
DOIs	https://doi.org/10.1107/S1744309106031216
Publication status	Published - 2006

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title = "Crystallization of mutant forms of glutaredoxin Grx1p from yeast",

abstract = "Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 {\AA} (synchrotron radiation) and 2.7 {\AA} (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO4 which diffract synchrotron radiation to 2.7 {\AA}.",

author = "H{\aa}kansson, {Kjell Ove} and Henrik {\O}stergaard and Winther, {Jakob R.}",

note = "glutaredoxins; Grx1p; yellow fluorescent protein.",

year = "2006",

doi = "10.1107/S1744309106031216",

language = "English",

volume = "62",

pages = "920--922",

journal = "Acta Crystallographica Section F: Structural Biology Communications",

issn = "2053-230X",

publisher = "Wiley",

number = "9",

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TY - JOUR

T1 - Crystallization of mutant forms of glutaredoxin Grx1p from yeast

AU - Håkansson, Kjell Ove

AU - Østergaard, Henrik

AU - Winther, Jakob R.

N1 - glutaredoxins; Grx1p; yellow fluorescent protein.

PY - 2006

Y1 - 2006

N2 - Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 Å (synchrotron radiation) and 2.7 Å (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO4 which diffract synchrotron radiation to 2.7 Å.

AB - Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 Å (synchrotron radiation) and 2.7 Å (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO4 which diffract synchrotron radiation to 2.7 Å.

U2 - 10.1107/S1744309106031216

DO - 10.1107/S1744309106031216

M3 - Journal article

SN - 2053-230X

VL - 62

SP - 920

EP - 922

JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

IS - 9

ER -

Crystallization of mutant forms of glutaredoxin Grx1p from yeast

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