TY - JOUR
T1 - Crystallization of mutant forms of glutaredoxin Grx1p from yeast
AU - Håkansson, Kjell Ove
AU - Østergaard, Henrik
AU - Winther, Jakob R.
N1 - glutaredoxins; Grx1p; yellow fluorescent protein.
PY - 2006
Y1 - 2006
N2 - Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 Å (synchrotron radiation) and 2.7 Å (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO4 which diffract synchrotron radiation to 2.7 Å.
AB - Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 Å (synchrotron radiation) and 2.7 Å (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO4 which diffract synchrotron radiation to 2.7 Å.
U2 - 10.1107/S1744309106031216
DO - 10.1107/S1744309106031216
M3 - Journal article
SN - 2053-230X
VL - 62
SP - 920
EP - 922
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
IS - 9
ER -