Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster

Tina Reinholdt Vognsen; Ole Kristensen

doi:10.1016/j.bbrc.2012.02.140

Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster

Tina Reinholdt Vognsen, Ole Kristensen

9 Citations (Scopus)

Abstract

The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7. å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-helices forming a cone-like shape. However, known binding sites for RanGDP and FxFG containing peptides show electrostatic and steric differences compared to nuclear transport factor 2. A HEPES molecule bound in the structure suggests a new, and possibly physiologically relevant, ligand binding site.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	420
Issue number	1
Pages (from-to)	188-92
Number of pages	5
ISSN	0006-291X
DOIs	https://doi.org/10.1016/j.bbrc.2012.02.140
Publication status	Published - 30 Mar 2012

Keywords

Amino Acid Sequence
Animals
Carrier Proteins
Crystallography, X-Ray
Drosophila Proteins
HEPES
Molecular Sequence Data
Nucleocytoplasmic Transport Proteins
Protein Structure, Secondary
Protein Structure, Tertiary

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title = "Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster",

abstract = "The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7. {\aa} resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-helices forming a cone-like shape. However, known binding sites for RanGDP and FxFG containing peptides show electrostatic and steric differences compared to nuclear transport factor 2. A HEPES molecule bound in the structure suggests a new, and possibly physiologically relevant, ligand binding site.",

keywords = "Amino Acid Sequence, Animals, Carrier Proteins, Crystallography, X-Ray, Drosophila Proteins, HEPES, Molecular Sequence Data, Nucleocytoplasmic Transport Proteins, Protein Structure, Secondary, Protein Structure, Tertiary",

author = "Vognsen, {Tina Reinholdt} and Ole Kristensen",

year = "2012",

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doi = "10.1016/j.bbrc.2012.02.140",

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journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster

AU - Vognsen, Tina Reinholdt

AU - Kristensen, Ole

PY - 2012/3/30

Y1 - 2012/3/30

N2 - The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7. å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-helices forming a cone-like shape. However, known binding sites for RanGDP and FxFG containing peptides show electrostatic and steric differences compared to nuclear transport factor 2. A HEPES molecule bound in the structure suggests a new, and possibly physiologically relevant, ligand binding site.

AB - The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7. å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-helices forming a cone-like shape. However, known binding sites for RanGDP and FxFG containing peptides show electrostatic and steric differences compared to nuclear transport factor 2. A HEPES molecule bound in the structure suggests a new, and possibly physiologically relevant, ligand binding site.

KW - Amino Acid Sequence

KW - Animals

KW - Carrier Proteins

KW - Crystallography, X-Ray

KW - Drosophila Proteins

KW - HEPES

KW - Molecular Sequence Data

KW - Nucleocytoplasmic Transport Proteins

KW - Protein Structure, Secondary

KW - Protein Structure, Tertiary

U2 - 10.1016/j.bbrc.2012.02.140

DO - 10.1016/j.bbrc.2012.02.140

M3 - Journal article

C2 - 22414690

SN - 0006-291X

VL - 420

SP - 188

EP - 192

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster

Abstract

Keywords

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