TY - JOUR
T1 - Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster
AU - Vognsen, Tina Reinholdt
AU - Kristensen, Ole
N1 - Copyright © 2012 Elsevier Inc. All rights reserved.
PY - 2012/3/30
Y1 - 2012/3/30
N2 - The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7. å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-helices forming a cone-like shape. However, known binding sites for RanGDP and FxFG containing peptides show electrostatic and steric differences compared to nuclear transport factor 2. A HEPES molecule bound in the structure suggests a new, and possibly physiologically relevant, ligand binding site.
AB - The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7. å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-helices forming a cone-like shape. However, known binding sites for RanGDP and FxFG containing peptides show electrostatic and steric differences compared to nuclear transport factor 2. A HEPES molecule bound in the structure suggests a new, and possibly physiologically relevant, ligand binding site.
KW - Amino Acid Sequence
KW - Animals
KW - Carrier Proteins
KW - Crystallography, X-Ray
KW - Drosophila Proteins
KW - HEPES
KW - Molecular Sequence Data
KW - Nucleocytoplasmic Transport Proteins
KW - Protein Structure, Secondary
KW - Protein Structure, Tertiary
U2 - 10.1016/j.bbrc.2012.02.140
DO - 10.1016/j.bbrc.2012.02.140
M3 - Journal article
C2 - 22414690
SN - 0006-291X
VL - 420
SP - 188
EP - 192
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -