Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII

TY - JOUR

T1 - Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII

AU - Engberg, J

AU - Nielsen, Henrik

N1 - Keywords: Animals; Base Sequence; DNA, Ribosomal; Extrachromosomal Inheritance; Molecular Sequence Data; Sequence Homology, Nucleic Acid; Tetrahymena; Transcription, Genetic

PY - 1990

Y1 - 1990

N2 - The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.

AB - The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.

M3 - Journal article

C2 - 2263454

SN - 0305-1048

VL - 18

SP - 6915

EP - 6919

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 23

ER -