Comparison of polyacetylene content in organically and conventionally grown carrots using a fast ultrasonic liquid extraction method

Malene Søltoft; John Nielsen; Kristian Holst Laursen; Søren Husted; Ulrich Halekoh; Pia Knuthsen

doi:10.1021/jf101921v

Comparison of polyacetylene content in organically and conventionally grown carrots using a fast ultrasonic liquid extraction method

Malene Søltoft, John Nielsen, Kristian Holst Laursen, Søren Husted, Ulrich Halekoh, Pia Knuthsen

35 Citations (Scopus)

Abstract

A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)based methodology using high-performance liquid chromatography-ultraviolet (HPLC-UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3-3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 μg of falcarindiol equiv/g of dry weight, respectively, and 3-15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year-year variation was observed for falcarindiol-3-acetate.

Original language	English
Journal	Journal of Agricultural and Food Chemistry
Volume	58
Pages (from-to)	7673-7679
Number of pages	7
ISSN	0021-8561
DOIs	https://doi.org/10.1021/jf101921v
Publication status	Published - 14 Jul 2010

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10.1021/jf101921v

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Comparison of polyacetylene content in organically and conventionally grown carrots using a fast ultrasonic liquid extraction method. / Søltoft, Malene; Nielsen, John ; Laursen, Kristian Holst et al.

In: Journal of Agricultural and Food Chemistry, Vol. 58, 14.07.2010, p. 7673-7679.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Comparison of polyacetylene content in organically and conventionally grown carrots using a fast ultrasonic liquid extraction method",

abstract = "A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)based methodology using high-performance liquid chromatography-ultraviolet (HPLC-UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3-3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 μg of falcarindiol equiv/g of dry weight, respectively, and 3-15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year-year variation was observed for falcarindiol-3-acetate.",

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T1 - Comparison of polyacetylene content in organically and conventionally grown carrots using a fast ultrasonic liquid extraction method

AU - Søltoft, Malene

AU - Nielsen, John

AU - Laursen, Kristian Holst

AU - Husted, Søren

AU - Halekoh, Ulrich

AU - Knuthsen, Pia

PY - 2010/7/14

Y1 - 2010/7/14

N2 - A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)based methodology using high-performance liquid chromatography-ultraviolet (HPLC-UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3-3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 μg of falcarindiol equiv/g of dry weight, respectively, and 3-15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year-year variation was observed for falcarindiol-3-acetate.

AB - A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)based methodology using high-performance liquid chromatography-ultraviolet (HPLC-UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3-3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 μg of falcarindiol equiv/g of dry weight, respectively, and 3-15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year-year variation was observed for falcarindiol-3-acetate.

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DO - 10.1021/jf101921v

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SN - 0021-8561

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SP - 7673

EP - 7679

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

ER -

Comparison of polyacetylene content in organically and conventionally grown carrots using a fast ultrasonic liquid extraction method

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