Comparative study of Gymnodinium mikimotoi and Gymnodinium aureolum comb. nov. (= Gyrodinium aureolum) based on morphology, pigment composition, and molecular data.

TY - JOUR

T1 - Comparative study of Gymnodinium mikimotoi and Gymnodinium aureolum comb. nov. (= Gyrodinium aureolum) based on morphology, pigment composition, and molecular data.

AU - Hansen, Gert

AU - Daugbjerg, Niels

AU - Henriksen, Peter

N1 - KEYWORDS accessory pigments • Gymnodinium aureolum • Gymnodinium mikimotoi • Gyrodinium aureolum • LSU rDNA, molecular phylogeny • taxonomy

PY - 2000

Y1 - 2000

N2 - Light and electron microscopy, nuclear-encoded LSU rDNA sequences, and pigment analyses were performed on five geographically separate isolates of Gymnodinium mikimotoi. The morphological variation between the isolates equals that found within the isolates. The nuclear-encoded LSU rDNA sequences were nearly identical in all isolates, and molecular analyses using maximum likelihood, parsimony, and neighbor joining showed the geographical isolates as an unresolved clade. Based on the available data it is concluded that the European isolates, formerly identified as Gyrodinium aureolum, Gyrodinium cf. aureolum, or Gymnodinium cf. nagasakiense, are conspecific with the Japanese Gymnodinium mikimotoi. An isolate from the Pettaquamscutt River, USA, is suggested to represent what Hulburt (1957) described as Gyrodinium aureolum. The LSU rDNA sequence data and ultrastructural characters in this isolate closely resemble those of Gymnodinium fuscum, the type species of Gymnodinium, and Gyrodinium aureolum Hulburt is therefore renamed Gymnodinium aureolum (Hulburt) G. Hansen, comb. nov.

AB - Light and electron microscopy, nuclear-encoded LSU rDNA sequences, and pigment analyses were performed on five geographically separate isolates of Gymnodinium mikimotoi. The morphological variation between the isolates equals that found within the isolates. The nuclear-encoded LSU rDNA sequences were nearly identical in all isolates, and molecular analyses using maximum likelihood, parsimony, and neighbor joining showed the geographical isolates as an unresolved clade. Based on the available data it is concluded that the European isolates, formerly identified as Gyrodinium aureolum, Gyrodinium cf. aureolum, or Gymnodinium cf. nagasakiense, are conspecific with the Japanese Gymnodinium mikimotoi. An isolate from the Pettaquamscutt River, USA, is suggested to represent what Hulburt (1957) described as Gyrodinium aureolum. The LSU rDNA sequence data and ultrastructural characters in this isolate closely resemble those of Gymnodinium fuscum, the type species of Gymnodinium, and Gyrodinium aureolum Hulburt is therefore renamed Gymnodinium aureolum (Hulburt) G. Hansen, comb. nov.

U2 - 10.1046/j.1529-8817.2000.99172.x

DO - 10.1046/j.1529-8817.2000.99172.x

M3 - Journal article

SN - 0022-3646

VL - 36

SP - 394

EP - 410

JO - Journal of Phycology

JF - Journal of Phycology

IS - 2

ER -