TY - JOUR
T1 - Comparative study of Gymnodinium mikimotoi and Gymnodinium aureolum comb. nov. (= Gyrodinium aureolum) based on morphology, pigment composition, and molecular data.
AU - Hansen, Gert
AU - Daugbjerg, Niels
AU - Henriksen, Peter
N1 - KEYWORDS
accessory pigments • Gymnodinium aureolum • Gymnodinium mikimotoi • Gyrodinium aureolum • LSU rDNA, molecular phylogeny • taxonomy
PY - 2000
Y1 - 2000
N2 - Light and electron microscopy, nuclear-encoded LSU rDNA sequences, and pigment analyses were performed on five geographically separate isolates of Gymnodinium mikimotoi. The morphological variation between the isolates equals that found within the isolates. The nuclear-encoded LSU rDNA sequences were nearly identical in all isolates, and molecular analyses using maximum likelihood, parsimony, and neighbor joining showed the geographical isolates as an unresolved clade. Based on the available data it is concluded that the European isolates, formerly identified as Gyrodinium aureolum, Gyrodinium cf. aureolum, or Gymnodinium cf. nagasakiense, are conspecific with the Japanese Gymnodinium mikimotoi. An isolate from the Pettaquamscutt River, USA, is suggested to represent what Hulburt (1957) described as Gyrodinium aureolum. The LSU rDNA sequence data and ultrastructural characters in this isolate closely resemble those of Gymnodinium fuscum, the type species of Gymnodinium, and Gyrodinium aureolum Hulburt is therefore renamed Gymnodinium aureolum (Hulburt) G. Hansen, comb. nov.
AB - Light and electron microscopy, nuclear-encoded LSU rDNA sequences, and pigment analyses were performed on five geographically separate isolates of Gymnodinium mikimotoi. The morphological variation between the isolates equals that found within the isolates. The nuclear-encoded LSU rDNA sequences were nearly identical in all isolates, and molecular analyses using maximum likelihood, parsimony, and neighbor joining showed the geographical isolates as an unresolved clade. Based on the available data it is concluded that the European isolates, formerly identified as Gyrodinium aureolum, Gyrodinium cf. aureolum, or Gymnodinium cf. nagasakiense, are conspecific with the Japanese Gymnodinium mikimotoi. An isolate from the Pettaquamscutt River, USA, is suggested to represent what Hulburt (1957) described as Gyrodinium aureolum. The LSU rDNA sequence data and ultrastructural characters in this isolate closely resemble those of Gymnodinium fuscum, the type species of Gymnodinium, and Gyrodinium aureolum Hulburt is therefore renamed Gymnodinium aureolum (Hulburt) G. Hansen, comb. nov.
U2 - 10.1046/j.1529-8817.2000.99172.x
DO - 10.1046/j.1529-8817.2000.99172.x
M3 - Journal article
SN - 0022-3646
VL - 36
SP - 394
EP - 410
JO - Journal of Phycology
JF - Journal of Phycology
IS - 2
ER -