Colour development of immunogold-labelled antibodies for light microscopy.

P Fritz; J Hoenes; J Schenk; A Mischlinski; A Grau; J G Saal; H V Tuczek; H Multhaupt; G Pfleiderer

Colour development of immunogold-labelled antibodies for light microscopy.

P Fritz, J Hoenes, J Schenk, A Mischlinski, A Grau, J G Saal, H V Tuczek, H Multhaupt, G Pfleiderer

7 Citations (Scopus)

Abstract

We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.

Original language	English
Journal	Histochemistry
Volume	85
Issue number	3
Pages (from-to)	209-14
Number of pages	5
ISSN	0301-5564
Publication status	Published - 1986
Externally published	Yes

Cite this

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title = "Colour development of immunogold-labelled antibodies for light microscopy.",

abstract = "We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.",

author = "P Fritz and J Hoenes and J Schenk and A Mischlinski and A Grau and Saal, {J G} and Tuczek, {H V} and H Multhaupt and G Pfleiderer",

note = "Keywords: Antibodies; Antigens, Surface; Arthritis, Rheumatoid; Gold; Humans; Immunoenzyme Techniques; Microscopy; Osteoarthritis; Synovial Membrane; T-Lymphocytes; Tonsil",

year = "1986",

language = "English",

volume = "85",

pages = "209--14",

journal = "Histochemistry",

issn = "0301-5564",

publisher = "Springer",

number = "3",

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TY - JOUR

T1 - Colour development of immunogold-labelled antibodies for light microscopy.

AU - Fritz, P

AU - Hoenes, J

AU - Schenk, J

AU - Mischlinski, A

AU - Grau, A

AU - Saal, J G

AU - Tuczek, H V

AU - Multhaupt, H

AU - Pfleiderer, G

N1 - Keywords: Antibodies; Antigens, Surface; Arthritis, Rheumatoid; Gold; Humans; Immunoenzyme Techniques; Microscopy; Osteoarthritis; Synovial Membrane; T-Lymphocytes; Tonsil

PY - 1986

Y1 - 1986

N2 - We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.

AB - We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.

M3 - Journal article

C2 - 3528080

SN - 0301-5564

VL - 85

SP - 209

EP - 214

JO - Histochemistry

JF - Histochemistry

IS - 3

ER -

Colour development of immunogold-labelled antibodies for light microscopy.

Abstract

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